Other

Centre National De La Recherche Scientifique

Universitë De Bordeaux

Imagine Optic

Bockelmann, Ulrich

Virgile Viasnoff

Xavier Levecq

Vincent Studer

Ulrich Bockelmann

Jean-Baptiste Sibarita

Ismaïl Cisse

Rémi Galland

According to one aspect, the invention concerns a method for microscopy of a thick sample arranged on a sample support, with edge-illumination of the sample. The method comprises, in particular, emitting at least one illumination beam (), forming, from the illumination beam, an illumination surface, focusing the illumination surface in the sample by means of a microscope lens () and deflecting the illumination surface originating from the microscope lens, in order to form a transverse illumination surface, located in a plane substantially perpendicular to the optical axis of the microscope lens. The method further comprises forming, by means of said microscope lens (), the image of an area of the sample illuminated by the transverse illumination surface on a detection surface () of a detection device (), scanning the illumination beam, allowing the transverse illumination surface to move along the optical axis of the microscope lens, and superimposing the object imaging surface and the transverse illumination surface, by focusing means comprising means separate from the means for the relative axial movement of the microscope lens and the sample.

System and method of edge-illumination microscopy

The present invention relates to a method for detecting a circularized single-stranded DNA by means of the isothermal hyperbranched rolling circle amplification technique, in which the primers used comprise a detectable barcode sequence and, optionally, a spacer which blocks polymerization by DNA polymerase. The present invention also relates to the use of said method for detecting a genetic polymorphism of one or more base pair(s).

Method for detecting a circularized DNA, and use of said method for detecting mutations

Growing community of inventors

Vanves, France

Virgile Viasnoff