Becton Dickinson and Company Limited

Virendra O Shah

Michael R Loken

Curt I Civin

Thomas J Mercolino

Leon W Terstappen

This invention describes methods and kits for determining instrument linearity of a flow cytometer and is particularly useful as a control particle for use in conjunction with absolute cell counting methods. The particle used in the practice of this invention comprises a small fluorescent bead.

Control particles for cell counting and instrument linearity

A method and kit for identification of the lineages and stages of hematopoietic cells in a sample wherein the cells are treated with labeled monoclonal antibodies selected to distinguish between each lineage and maturational stages within each lineage.

Method to determine the composition of bone marrow samples

A method is disclosed for the discrimination between intact and damaged cells in a sample, and more particularly, the method uses a vital nucleic acid dye to selectively stain intact versus damaged cells which then may be counted and sorted by flow cytometry means. The method also may be used in conjunction with fluorescently labelled monoclonal antibodies to simultaneously identify cellular antigens.

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Bernard Township, NJ, United States of America

Virendra O Shah