US Government as Represented by the Secretary of the Army

Vipin K Rastogi

Tu-Chen Cheng

Joseph J DeFrank

Steven P Harvey

Michael F Kauzlarich

Amee L Polk

Nino L Bonavito

Lisa S Smith

A biocidal and sporicidal smoke composition(s) using iodine oxide and metal powder is provided. This composition generates iodine gas or smoke as the primary biocidal agent, as well as metal oxides that provide additional biocidal properties. The smoke producing composition is suitable for pressing into canisters of compacted powder at a load pressure range of 1500 to 7500 psi and can be used for decontamination of spaces believed to be contaminated with biological agents. The composition is also suitable for use in hand grenades, smoke pots, rifle grenades, mortars, multiple launch grenades, shoulder fired missiles, and artillery rounds, as well as first responder and commercial biological decontamination applications.

Pyrotechnic iodine smoke generation for counter biological applications

Pyrotechnic iodine smoke generation for counter biological application

The present invention relates to a near-universal non-corrosive, non-toxic, environmentally safe and user friendly decontaminant capable of detoxifying organophosphorus (OP)-based G-type, V-type neurotoxic chemical warfare, sulfur-mustard, and related OP based hazardous industrial materials in a dry powder form. The decontaminant contains OPH enzyme, OPAA enzyme, DFPase enzyme, dehalogenase enzyme, quaternary ammonium salt, a pH control reagent, a fire-fighting agent, and a foaming agent. The decontaminant is mixed with available water for use.

Non-corrosive, non-caustic, non-flammable, catalyst-based decontaminant formulation

The invention provides purified and isolated DNA fragments fromchromosomal DNA, primer sets and probes derived therefrom, as well as kits and detection methods for. The methods of the invention provide for specific detection of anthrax over closely related strains of, as well as accurate detection of low numbers ofin an environmental sample containing large amounts of non-specific DNA. The invention is applicable to food, health care, and military applications.

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Nucleotide sequences for detection of bacillus anthracis

Nucleotide sequences for detection of Bacillus anthracis

The invention provides purified and isolated DNA fragments from chromosomal DNA, primer sets and probes derived therefrom, as well as kits and detection methods for . The methods of the invention provide for specific detection of anthrax over closely related strains of , as well as accurate detection of low numbers of in an environmental sample containing large amounts of non-specific DNA. The invention is applicable to food, health care, and military applications.

Novel and improved processes for isolating organophosphorus hydrolase enzyme from an aqueous solution and obtaining substantially purified enzyme at high yield are provided, as well as compositions, including storage stable lyophilyzed organophosphorus hydrolase enzyme compositions, that are prepared by the provided methods. The organophosphorus hydrolase enzyme is purified by contacting an aqueous solution of cell free bacterial proteins with a strong cation exchange resin, the aqueous solution comprising soluble organophosphorus hydrolase enzyme, washing the strong cation exchange resin with a washing buffer to remove unbound proteins from the strong cation exchange resin, eluting proteins that remain bound to the strong cation exchange resin by washing the resin with an eluting buffer comprising salt in a concentration that starts at about zero and is raised during the eluting process to about 0.5M, and detecting and collecting eluate comprising a protein having organophosphorus hydrolase enzyme activity. All processing steps are conducted at a pH that is less than the isoelectric point of the organophosphorus hydrolase enzyme.

One-step purification process for organophosphorus hydrolase enzyme

The invention provides purified and isolated DNA fragments from chromosomal DNA, primer sets and probes derived therefrom, as well as kits and detection methods for The methods of the invention provide for specific detection of anthrax over closely related strains of Bacillus, as well as accurate detection of low numbers of in an environmental sample containing large amounts of non-specific DNA. The invention is applicable to food, health care, and military applications.

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Bel Air, MD, United States of America

Vipin K Rastogi