Stellenbosch University

Lallemand Hungary Liquidity Management LLC

Danstar Ferment Ag

Universiteit Stellenbosch

Vineet B Rajgarhia

Aaron Argyros

Trisha Barrett

Nicky Caiazza

John E McBride

Allan C Froehlich

Charles F Rice

Erin Wiswall

Mark Mellon

Ryan Skinner

Anne K Warner

Emily Stonehouse

Riaan Den Haan

Shital A Tripathi

Haowen Xu

Chhayal V Gandhi

Kristen M Deleault

John S Bardsley

Frank Agbogbo

Kevin S Wenger

Indraneel Shikhare

Alan Belcher

Abigail Foster

Jennifer Gosselin

Yin-Ying Chiu

Elena E Brevnova

Heidi H Hau

Heidi Lau

Elena Brevnova

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

Growing community of inventors

Courbevoie, France

Vineet B Rajgarhia