Vivascience Ag

Sartorius Ag

Vincenzo Vassarotti

Colin Lanyi

A device for concentrating and/or purifying macromolecules in a solution has a concentration chamber ( ) with a sample reservoir ( ) for a liquid sample to be processed, a filter ( ) for filtering the liquid sample, and a filtrate container ( ) for collecting the filtrate. A gas pressure differential is created and maintained across the filter and a sealing cap ( ) for the sample reservoir ( ), which has an opening ( ) for permitting gas flow in one direction and blocking aerosol or liquid flow in an opposed direction. The present invention also provides apparatus and methods employing the device.

Method, device and apparatus for concentrating and/or purifying macromolecules in a solution

A device for removing or replacing solvent in a liquid sample containing macromolecules includes a concentration chamber having an aperture adjacent to the upper end of the concentration chamber which is arranged to receive and tightly fix the outlet of a detachable auxiliary reservoir. In methods employing the device, the concentration chamber is filled with part of a liquid sample to a predetermined level, and a detachable auxiliary upper reservoir containing the rest of the liquid sample is tightly fixed to the aperture adjacent to the upper end of the concentration chamber to successively deliver additional liquid sample as the concentration is progressing, or a detachable auxiliary upper reservoir containing a solvent or buffer solution is tightly fixed to the aperture adjacent to the upper end of the concentration chamber to successively deliver solvent or buffer solution as a replacement for the liquid absorbed by an absorption material.

Method for concentrating or washing macromolecules in a solution and device for carrying out said method

A filtration cell for carrying out a tangential flow filtration of a sample liquid contains at least two blocks and at least two filtration elements arranged between each pair of two adjacent blocks. The filtration elements each include an outlet for filtrate, a sheet of support material and two generally flat ultrafiltration or microfiltration membranes, arranged on either side of the support material in a sandwich construction. Each of the blocks, at a side adjacent the membranes, is provided with a respective channel for feeding a flow of sample liquid tangentially over the membranes such that each channel is connected in parallel with the inlet for the flow of sample liquid to be filtered and with an outlet for a concentrated solution. Each channel includes in its longitudinal direction a number of subsequent channel sections separated by transitional zones and is constructed and arranged such that the main flow direction in subsequent sections changes abruptly in the transitional zones.

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Bugnaux sur Rolle, Switzerland

Vincenzo Vassarotti