The General Hospital Corporation

Massachusetts General Hospital Corporation

Centocor, Inc.

Other

Alnylam Pharmaceuticals, Inc.

Vincent R Zurawski, Jr

Jack Raymond Wands

Takaji Wakita

Catherine Julia Pachuk

Hubert J Schoemaker

Leslie R Coney

Theodore John Nitz

David M Stout

Liat Mintz

Moussa B H Youdim

C Satishchandran

Orly Weinreb

Hugh M Davis

Thomas L Klug

Schoemaker, Hubert J P

Barbara L Westrick

8-Hydroxy-quinoline derivatives and 8-ethers, 8-esters, 8-carbonates, 8-acyloxymethyl, 8-phosphates, (phosphoryloxy)methyl, and 8-carbamates derivatives thereof are described that exhibit iron chelation, neuroprotective, neurorestorative, apoptotic and/or selective MAO-AB inhibitory activities.

Neuroprotective and neuro-restorative iron chelators and monoamine oxidase inhibitors and uses thereof

Nucleic acid molecule that comprise an incomplete hepatitis C viral genome are provided. Pharmaceutical compositions that contain nucleic acid molecules comprising an incomplete hepatitis C viral genome including a nucleotide sequence encoding a complete hepatitis C core protein operably linked to regulatory elements functional in human cells are provided. Methods of immunizing individuals susceptible to or infected by hepatitis C virus comprising the step of administering such pharmaceutical compositions are provided.

Hepatitis C virus vaccine

Nucleic acid molecule that comprise an incomplete hepatitis C viral genome including specifically disclosed DNA sequences are disclosed. Pharmaceutical compositions that contain nucleic acid molecules comprising an incomplete hepatitis C viral genome including a nucleotide sequence encoding a complete hepatitis C core protein operably linked to regulatory elements functional in human cells are disclosed. Methods of immunizing individuals susceptible to or infected by hepatitis C virus comprising the step of administering such pharmaceutical compositions are disclosed.

Hybridomally produced monoclonal IgM antibodies having high affinity are useful for the immunoassay and purification of vital antigens.

Immunoassay utilizing monoclonal high affinity IgM antibodies

A preparation of an isolated immunoreactive CA 125 Antigen, and a method of isolating it is disclosed. CA 125 Antigen is a glycoprotein having a molecular weight of about 200kD, and a carbohydrate-content of about 24%. The CA 125 Antigen is isolated from a cell culture medium by acid precipitation, and is subsequently purified by size exclusion chromatography and immunoaffinity chromatography.

Method of isolating CA 125 antigen

The monoclonal antibodies are specific for a determinant found on hepatitis B surface antigen, and show high affinity for this determinant. Hybridomally produced monoclonal IgM antibodies having high affinity are useful for the immunoassay and purification of viral antigens.

Monoclonal IgM antibody with specificity against hepatitis B surface

Hybridomally produced monoclonal IgM antibodies having high affinity are useful for the immunoassay and purification of viral antigens.

A simultaneous sandwich immunoassay employing high-affinity monoclonal antibodies is disclosed. This simultaneous sandwich assembly has surprising sensitivity compared to forward and reverse sandwich assays for the detection of multi-determinant antigens such as hepatitis B surface antigen.

Immunoassay for multi-determinant antigens using high-affinity

Cell lines for producing monoclonal antibodies to hepatitis virus are established by immunizing animal lymphocytes with hepatitis antigen to form antibody producing cells which then are fused with myeloma cells. The resultant somatic cell hybrids can be cloned. These clones produce monoclonal antibodies to individual antigenic determinates unique to hepatitis virus.

Process for producing antibodies to hepatitis virus and cell lines

Human peripheral blood lymphocytes are stimulated with either a polyclonal activator or an antigen which gives rise to immunologically activated B lymphocytes. These activated B lymphocytes are separated from non-activated B lymphocytes by relying on the fact that activated B lymphocytes possess a surface marker; the ability to rosette Rhesus monkey lymphocytes (RhMRBC), not found on their non-activated counterparts. Separation is achieved by rosetting with RhMRBC and subsequent density centrifugation of cells.

Growing community of inventors

West Chester, PA, United States of America

Vincent R Zurawski, Jr