Illumina Cambridge Limited

Illumina Corporation

Other

Cambridge University Technical Services Limited

Vincent Peter Smith

Jonathan Mark Boutell

Roberto Rigatti

Raquel Maria Sanches-Kuiper

Sean Humphray

Niall Anthony Gormley

Geoffrey Paul Smith

Colin Lloyd Barnes

Tobias William Barr Ost

Eric Hans Vermaas

David Bentley

Graham John Worsley

Min-Jui Richard Shen

Kay Klausing

John Moore

Kevin Hall

Isabelle Marie Julia Rasolonjatovo

Claire Bevis-Mott

Sohela De Rozieres

Stacey Efstathiou

Avgousta Ioannou

Antonio Alcami

Angela Kalbande

Antonio Alcami Alcami Pertejo

Michael Chesney

João Pedro Monteiro E Louro Machado De Simas

Christopher Marc Parry

Margarida Saraiva

Yin Ho

Maria Begona Ruiz-Arguello

Ali Alejo Herberg

Isabelle Marie Rasolonjatovo

Epameinondas Fritzilas

Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.

Methods and compositions for preparing nucleic acid libraries

The present invention relates to methods of imaging template hybridisation for estimating cluster numbers prior to solid phase amplification and sequencing. More particularly, an initial round of imaging is carried out at the single molecule template hybridisation stage which allows a general estimation of cluster numbers prior to clusters being formed. Amplification of the signal allows single molecule imaging to be carried out using standard sequencing imaging apparatus.

Methods for estimating cluster numbers

The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by exonuclease treatment and optionally blocking the 3' ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing.

Compositions and methods for improving sample identification in indexed nucleic acid libraries

The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by blocking the 3' ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing.

The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.

Method for sequencing a polynucleotide template

Embodiments disclosed herein provide reagents and kits for nucleic acid preparation comprising a siderophore. Embodiments disclosed herein provide methods for preparing a nucleic acid library, which comprise: providing a plurality of nucleic acid molecules from a sample; and manipulating the plurality of nucleic acid molecules in a reagent for nucleic acid preparation comprising a siderophore. Further, embodiments disclosed herein provide methods for reducing oxidative damage to a nucleic acid molecule or increasing the Q (phred) score of a sequencing reaction, which methods comprise preparing the nucleic acid molecule in the absence of EDTA.

Reducing DNA damage during sample preparation and sequencing using siderophore chelators

A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

Method for nucleotide detection

Method for sequencing a polynucelotide template

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Nr Saffron Walden, United Kingdom

Vincent Peter Smith