Rigel Pharmaceuticals, Incorporated

Qiagen Mansfield, Inc.

Vincent Miles

Michael G Katze

Michael B Mathews

Julia C Watson

Gary Witherell

Jork Nolling

Described herein are approaches for the detection, identification, and/or quantification of target nucleic acids, including, but not limited to partially, substantially randomly, degraded target nucleic acids, in a biological sample, such as a formalin-fixed, paraffin-embedded (FFPE) sample. These approaches provide a means of detecting, identifying, and/or quantifying target nucleic acid molecules, including DNA and RNA molecules, further including RNAs of different classes, from the same sample, and in the same reaction, by using 'expander oligonucleotides,' as the term is defined herein, to convert fragments of target nucleic acids into discretely sized DNA fragments, each with a chosen length characteristic for the target nucleic acid from which it is derived.

Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample

Method for screening for an antiviral agent, by determining whether a potential agent interacts with a virus or cellular component which allows or prevents preferential translation of a virus RNA compared to a host RNA under virus infection conditions; and determining whether any interaction of the agent with the component reduces the level of translation of an RNA of the virus.

Method for selective inactivation of viral replication

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Chestnut Hill, MA, United States of America

Vincent Miles