Rijk Zwaan Zaadteelt En Zaadhandel B.v.

Vincent Laurens Adrianus Kock

Johannes Geert Jan Feitsma

Raoul Jacobus Johannes Maria Frijters

Jan Hugo Den Braber

The present invention relates to an allele designated alpha-CMV which confers resistance to CMV when present in a spinach plant, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif 'MAEIGYSVC' (SEQ ID NO: 22) at its N-terminus; and b) the motif “KWMCLR” (SEQ ID NO: 23); and c) an LRR domain that has in order of increased preference at least 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID NO: 10. The allele has a genomic nucleotide sequence which in order of increased preference has at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID NO: 1. The invention further relates to plants comprising the allele and to various methods involving the allele.

CMV resistance allele

The invention relates to plants and plant parts, in particular spinach plants (.), which are resistant tof. sp.. The invention also relates to seeds capable of producingf. sp.resistant plants. The invention further relates to methods for obtaining said plants with altered genotypes and seeds thereof, which are resistant tof sp.

Non R-gene mediated resistance

The present invention relates to an allele designated alpha-WOLF 25 which confers resistance to at least onef. sp.race, wherein the protein encoded by said allele is a CC-NB S-LRR protein that comprises in its amino acid sequence: a) the motif 'MAEIGYSVC' at its N-terminus; and b) the motif 'KWMCLR'; and wherein the LRR domain of the protein has in order of increased preference at least 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID NO: 5. The allele when present in a spinach plant confers complete resistance to at leastf sp.race Pfs:8, Pfs15 and Pfs:16, and does not confer resistance to Pfs:3.

resistance in

The present invention relates to a method for modifying the resistance profile of a spinach plant tof sp., comprising introducing a WOLF allele or a resistance-conferring part thereof into the genome of said spinach plant, or modifying an endogenous WOLF allele in the genome of said spinach plant. A WOLF allele encodes a CC-NBS-LRR protein that comprises in its amino acid sequence: the motif 'MAEIGYSVC' at its N-terminus, and the motif 'KWMCLR' for alpha-type WOLF proteins or &#x201c;HVGCVVDR&#x201d; for beta-type WOLF proteins. The invention relates to the WOLF alleles referred to in Table 3. The invention further provides a method for selecting a spinach plant comprising a novel WOLF gene that confers resistance tof sp.in a spinach plant and a method for identifying a WOLF allele that confers resistance to one or more pathogenic races off. sp.in a spinach plant and to primers for use in these methods.

Method for modifying the resistance profile ofto downy mildew

The present invention relates to a method for modifying the resistance profile of a spinach plant tof. sp., comprising introducing a WOLF allele or a resistance-conferring part thereof into the genome of said spinach plant, or modifying an endogenous WOLF allele in the genome of said spinach plant. The invention further provides a method for selecting a spinach plant comprising a novel WOLF gene that confers resistance tof. sp.in a spinach plant and a method for identifying a WOLF allele that confers resistance to one or more pathogenic races off. sp.in a spinach plant and to primers for use in these methods.

The present invention relates to an allele designated alpha-WOLF 8 which confers resistance to at least onef. Sp.race, wherein the protein encoded by said allele is a CC-NB S-LRR protein that comprises in its amino acid sequence: a) the motif 'MAEIGYSVC' (SEQ ID NO: 15) at its N-terminus; and b) the motif 'KWMCLR' (SEQ ID NO: 16); and wherein the LRR domain of the protein has in order of increased preference at least 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID NO: 12. When the allele is homozygously present in a spinach plant confers complete resistance tof. Sp.races pfs:1, pfs:2, pfs:6, pfs:8 and pfs:15, and isolate US1508, and confers intermediate resistance to pfs:5, pfs:10 and pfs:16, and does not confer resistance to pfs:3, pfs:4, pfs:7, pfs:9, pfs:11, pfs:12, pfs:13 and pfs:14.

The present invention relates to an allele designated alpha-WOLF 15 which confers resistance to at least onef. Sp.race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif 'MAEIGYSVC' (SEQ ID NO: 13) at its N-terminus; and b) the motif 'KWMCLR' (SEQ ID NO: 14); and wherein the LRR domain of the protein has in order of increased preference at least 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID NO: 10. The allele when homozygously present in a spinach plant confers complete resistance tof. Sp.races pfs:1, pfs:2, pfs:3, pfs:4 and pfs:5, pfs:6, pfs:8, pfs:9, pfs:11, pfs:12, pfs:13, pfs:14, pfs:15, pfs:16 and isolates UA1014 and US1508, and confers intermediate resistance to pfs:10, and does not confer resistance to pfs:7.

The present invention relates to an allele designated alpha-CMV which confers resistance to CMV when present in a spinach plant, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif 'MAEIGYSVC' (SEQ ID NO: 22) at its N-terminus; and b) the motif 'KWMCLR' (SEQ ID NO: 23); and c) an LRR domain that has in order of increased preference at least 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID NO: 10. The allele has a genomic nucleotide sequence which in order of increased preference has at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID NO: 1. The invention further relates to plants comprising the allele and to various methods involving the allele.

The present invention relates to an allele designated alpha-WOLF 8 which confers resistance to at least onef. sp.race, wherein the protein encoded by said allele is a CC-NB S-LRR protein that comprises in its amino acid sequence: a) the motif 'MAEIGYSVC' (SEQ ID NO: 15) at its N-terminus; and b) the motif 'KWMCLR' (SEQ ID NO: 16); and wherein the LRR domain of the protein has in order of increased preference at least 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID NO: 12. When the allele is homozygously present in a spinach plant confers complete resistance tof. sp.races Pfs:1, Pfs:2, Pfs:6, Pfs:8 and Pfs:15, and confers intermediate resistance to Pfs:5, Pfs:10 and Pfs:16, and does not confer resistance to Pfs:3, Pfs:4, Pfs:7, Pfs:9, Pfs:11, Pfs:12, Pfs:13 and Pfs:14.

resistance in spinacia oleracea

The present invention relates to an allele designated alpha-WOLF 15 which confers resistance to at least onef sp.race, wherein the protein encoded by said allele is a CC-NB S-LRR protein that comprises in its amino acid sequence: a) the motif 'MAEIGYSVC' (SEQ ID NO: 13) at its N-terminus; and b) the motif 'KWMCLR' (SEQ ID NO: 14); and wherein the LRR domain of the protein has in order of increased preference at least 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100% sequence similarity to SEQ ID No:10. The allele when homozygously present in a spinach plant confers complete resistance tof sp.races Pfs:1, Pfs:2, Pfs:3, Pfs:4 and Pfs: 5, Pfs:6, Pfs:8, Pfs:9, Pfs:11, Pfs:12, Pfs:13, Pfs:14, Pfs:15, and isolates UA1014, and confers intermediate resistance to Pfs:10, and does not confer resistance to Pfs:7 and Pfs: 16.

Peronospora resistance in spinacia oleracea

The present invention relates to a method for modifying the resistance profile of a spinach plant tof. sp., comprising introducing a WOLF allele or a resistance-conferring part thereof into the genome of said spinach plant, or modifying an endogenous WOLF allele in the genome of said spinach plant. A WOLF allele encodes a CC-NBS-LRR protein that comprises in its amino acid sequence: the motif 'MAEIGYSVC' at its N-terminus, and the motif 'KWMCLR' for alpha-type WOLF proteins or &#x201c;HVGCVVDR&#x201d; for beta-type WOLF proteins. The invention relates to the WOLF alleles referred to in Table 3. The invention further provides a method for selecting a spinach plant comprising a novel WOLF gene that confers resistance tof. sp.in a spinach plant and a method for identifying a WOLF allele that confers resistance to one or more pathogenic races off. sp.in a spinach plant and to primers for use in these methods.

The invention relates to plants and plant parts, in particular spinach plants (L.), which are resistant tof. sp.. The invention also relates to seeds capable of producingf. sp.resistant plants. The invention further relates to methods for obtaining said plants with altered genotypes and seeds thereof, which are resistant tof. sp.

The present invention relates to a spinach plant which may comprise an allele conferring resistance to downy mildew, propagation material of said spinach plant, a cell of said spinach plant, seed of said spinach plant, and to harvested leaves of said spinach plant. The invention further relates to use of a spinach plant in breeding to confer resistance against downy mildew.

Growing community of inventors

De Lier, Netherlands

Vincent Laurens Adrianus Kock