The United States of America, As Represented by the Secretary, Department of Health and Human Services

Sandia Corporation

Vincent Hill

Jothikumar Narayanan

Blake A Simmons

Eric Bryant Cummings

Yolanda Fintschenko

Brian Holloway

Prithiviraj Jothikumar

This disclosure related to methods and reagents for isothermal amplification of nucleic acid molecules. In some embodiments, methods are provided for amplification of a nucleic acid molecule from a biological sample. Additional embodiments include identification of a target nucleic acid molecule in a biological sample using the disclosed amplification methods.

Methods and reagents for amplifying nucleic acids

This application provides photoinduced electron transfer (PET) nucleic acid molecules that can be used detect and amplify nucleic acid molecules, such as target nucleic acid molecules. These PET tags can be attached to the 5'-end of a target sequence-specific primer, thereby generating a PET primer. In particular examples, a PET tag includes a 5&#x2032;-labeled nucleotide that can be quenched by at least two consecutive Gs within the tag sequence, and is unquenched when the PET tag hybridizes with its complementary nucleic acid molecule. Also disclosed are methods of using PET primers in nucleic acid amplification, such as real-time PCR.

Photoinduced electron transfer (PET) primer for nucleic acid amplification

Disclosed is a method for monitoring sources of public water supply for a variety of pathogens by using a combination of ultrafiltration techniques together dielectrophoretic separation techniques. Because water-borne pathogens, whether present due to 'natural' contamination or intentional introduction, would likely be present in drinking water at low concentrations when samples are collected for monitoring or outbreak investigations, an approach is needed to quickly and efficiently concentrate and separate particles such as viruses, bacteria, and parasites in large volumes of water (e.g., 100 L or more) while simultaneously reducing the sample volume to levels sufficient for detecting low concentrations of microbes (e.g., &#x3c;10 mL). The technique is also designed to screen the separated microbes based on specific conductivity and size.

Method for concentration and separation of biological organisms by ultrafiltration and dielectrophoresis

Concentration and separation of biological organisms by ultrafiltration and dielectrophoresis

This application provides universal labeled primers for detection and amplification of nucleic acid molecules. These universal primers can be attached to the 5'-end of a target sequence-specific primer. In particular examples, the universal primer includes a labeled nucleotide flanked on both sides a nucleotide whose complement nucleotides changes a detectable signal from the label when the universal primer hybridizes with its complementary nucleic acid molecule. Also disclosed are methods of using the universal primer in nucleic acid amplification, such as real-time PCR.

Growing community of inventors

Decatur, GA, United States of America

Vincent Hill