Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Vincent Agache

Jean-Marc Fedeli

Yves Fouillet

Fabrice Casset

Patrice Caillat

Sebastien Hentz

Françoise Vinet

Stephane Fanget

Guillaume Delapierre

Pierre Puget

Francois Baleras

Stéphane Fanget

Olivier Constantin

Frédéric Revol-Cavalier

Antoine Hoang

Arnaud Millet

François Baleras

Leopold Virot

Gerald Blanco-Gomez

Eloise Pariset

Gérald Blanco-Gomez

A system for handling biological cells includes a main channel having an inlet end and an outlet end, and, at least over a first portion from the inlet end, including a cross section such that a cell circulating in the portion undergoes mechanical stresses. The system further includes first means for detecting the presence of a cell at the inlet end of the main channel and at least one access zone opening into the main channel between its inlet end and its outlet end in the first portion, in order to make it possible to exert an action on the cell. The system also includes means for displacing the cell in order to control the displacement of the cell between the inlet end and the outlet end.

Microfluidic system for handling biological cells

The invention is an electromechanical resonator, comprising a fixed portion and an oscillator oscillating at a resonant frequency and comprising a fluidic channel. The channel defines a fluidic circuit, can receive a fluid, and can be deformed at the resonant frequency. The resonator includes a waveguide, defining a photonic circuit, guiding a light wave between an input and an output of the waveguide and being able to be deformed at the resonant frequency. The waveguide input can be connected to a light source and the waveguide output can be connected to a photodetector able to form a signal representative of the light wave propagated by the waveguide towards the photodetector, the light wave being modulated at a frequency dependent on the resonant frequency. A variation in a mass of the fluid, inducing a variation in the resonant frequency, may be detected via the signal formed by the photodetector.

Optical and electromechanical resonator

The invention is a process for producing an electromechanical device including a movable portion that is able to deform with respect to a fixed portion. The process implements steps based on fabrication microtechnologies, applied to a substrate including an upper layer, an intermediate layer and a lower layer. These steps are:

Process for producing an electromechanical device

A detection method of detecting analytes of interest which are present in a liquid. The detection method including the steps of forming drops of liquid on a first surface by capillary breaking of a finger of liquid, which is initially formed by liquid dielectrophoresis. The thus formed drops each come into contact with a different detection surface, which is arranged facing the first surface. Analytes of interest which are present in each of the drops are detected at the corresponding detection surface.

Method and microsystem for detecting analytes which are present in drops of liquid

The invention relates to a device for mass detection of particles in a fluid medium, including an electromechanical oscillator (), excitation means () fitted so as to cause the said oscillator to vibrate, a fluid supply circuit (), and a fluid system () incorporated in the said oscillator, where the said fluid system () is in fluid communication with the fluid supply circuit (), where the said fluid system () includes at least one trapping site () configured to trap a particle of interest according to the size of the said particle of interest.

Device for mass detection of particles in a fluid medium, and implementation method

The present invention relates to a method for functionalizing fluid lines () in a micromechanical device, the walls of which include an opaque layer. For this purpose, the invention provides a method for functionalizing a micromechanical device provided with a fluid line including a peripheral wall () having a surface () outside the line and an inner surface () defining a space () in which a fluid can circulate, the peripheral wall at least partially including a silicon layer (). The method includes the following steps: a) providing a device, the peripheral wall () of which at least partially includes a silicon layer () having, at least locally, a thickness (e) of more than 100 nm and less than 200 nm, advantageously of 160 to 180 nm; c) silanizing at least the inner surface of the fluid line; d) the localized, selective photo-deprotection on at least the inner surface of the silanized device by exposing the peripheral wall () at the point at which said wall has a thickness (e) of more than 100 nm and less than 200 nm, advantageously of 160 to 180 nm.

Method for functionalising fluid lines contained in a micromechanical device, micromechanical device including functionalised lines, and method for manufacturing same

The invention provides a device for the gravimetric detection of particles in a fluid medium, simultaneously providing a high quality factor, easier operating conditions, small size and low production costs. For this purpose, the subject of the invention is a device for the gravimetric detection of particles in a fluid medium, comprising a flat electromechanical oscillator (), means for supporting the oscillator and means for actuating said oscillator, said means being designed to ensure that the oscillator () vibrates in its plane, said device further including a channel () for passage of the fluid, said channel being in fluid communication with a through-cavity () made in said oscillator.

Device for the gravimetric detection of particles in a fluid medium, comprising an oscillator over which a fluid stream flows, production process and method of employing the device

The invention provides a device for the gravimetric detection of particles in a fluid medium, comprising a flat electromechanical oscillator (), means for supporting the oscillator, means () for actuating said oscillator and, on either side of the plane of the oscillator (), two cavities () enabling the oscillator () to vibrate when it is activated by the actuation means (), characterized in that at least one of the two cavities () forms an integral part of a channel () for the passage of a fluid over at least one of the faces () of the oscillator and in that said actuation means () take the form of at least one electrode () lying in the same plane as that of the electromechanical oscillator and at a defined distance (g) from the oscillator, so as to ensure that the oscillator vibrates in its plane.

Device for the gravimetric detection of particles in a fluid medium, comprising an oscillator between two fluid channels

The invention relates to a detection device of small size, allowing direct in situ detection of particles with no labelling, enabling the particles to be rapidly analysed, and having both a specificity and a sensitivity that are at least equivalent to the existing devices. In one embodiment, the invention provides a device having a nanowire, intended for interacting with the particles of interest, which is suspended between two anchors that define a source and a drain, the source and the drain are configured to be connected to an AC voltage generator and to a DC voltage generator, respectively, in order to generate a first input signal; an excitation electrode, placed laterally facing the nanowire and configured to be connected to an AC voltage generator, in order to generate a second input signal; and a measurement electrode placed opposite the excitation electrode relative to the nanowire and generating a single output signal representative of the particles of interest.

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St Martin le Vinoux, France

Vincent Agache