Massachusetts Institute of Technology

The Broad Institute, Inc.

Dana-farber-cancer Institute Inc.

Harvard College

Liu, Yaping

Adalsteinsson, Viktor

Zhang, Zhizhuo

Kellis, Manolis

Viktor A Adalsteinsson

J Christopher Love

Todd Michael Gierahn

Denis Loginov

Alan Stockdale

Robert Samuel Langer

Klavs F Jensen

Armon R Sharei

Nahyun Cho

Gerassimos M Makrigiorgos

Robert S Langer, Jr

Manolis Kellis

Zhizhuo Zhang

Gavin Ha

Samuel Freeman

Yaping Liu

Described herein are systems, methods, and apparatus for automatically identifying and recovering individual cells of interest from a sample of biological matter, e.g., a biological fluid. Also described are methods of enriching a cell type of interest. These systems, methods, and apparatus allow for coordinated performance of two or more of the following, e.g., all with the same device, thereby enabling high throughput: cell enrichment, cell identification, and individual cell recovery for further analysis (e.g., sequencing) of individual recovered cells.

Systems, methods, and apparatus for in vitro single-cell identification and recovery

Isolating or identifying a cell based on a physical property of said cell can include providing a cell suspension; passing said suspension through a microfluidic channel that includes a constriction; passing the cell suspension through the constriction; and, contacting said cell suspension solution with a compound. The constriction can be sized to preferentially deform a relatively larger cell compared to a relatively smaller cell.

Selective delivery of material to cells

As described below, disclosed herein are methods of analyzing DNA methylation in cell-free DNA (cfDNA) and genomic DNA (gDNA) from sequencing data.

Methods for genome characterization

The invention provides methods of using low coverage sequencing to assess the relative fraction of tumor versus normal DNA in a sample, and to assess copy number alterations present in the sample.

Provided herein are methods to determine the original abundance of mutant alleles of one or more barcoded target sequences following mutation enrichment and sequencing.

Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection

Growing community of inventors

Wakefield, MA, United States of America

Viktor A Adalsteinsson