Sigma-aldrich Co. LLC

Vikas B Palhan

Carol A Kreader

Gregory D Davis

Carol Kreader

Ernie Mueller

The present disclosure provides reagents and methods for molecular proximity detection of specific endogenous nucleic acids in situ using RNA-guided nucleic acid binding proteins.

Methods and reagents for molecular proximity detection using RNA-guided nucleic acid binding proteins

Methods for visualizing modified nucleotides in a specific nucleic acid sequence or specific nucleic acid sequence interactions in single cells, wherein the methods comprise coupling an in situ hybridization (ISH) reaction with a proximity ligation assay (PLA) reaction.

Visualizing modified nucleotides and nucleic acid interactions in single cells

The present invention provides isolated, non-viable bacterial cells comprising a plurality of nucleic acid crosslinks, methods for making said cells, and methods for using said cells to isolate DNA-protein complexes. In particular, the nucleic acids of the cells are crosslinked by contacting the cells with a furocoumarin compound and ultraviolet light.

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St. Louis, MO, United States of America

Vikas B Palhan