Qiagen Gmbh

Sinvent As

Qiagen As

Vidar Skagestad

Lars Kilaas

Anne Dalager Dyrli

Arne Deggerdal

Evy Reitan

Magnetic particles capable of binding a target substance, which comprise a magnetic material and a matrix material, wherein the magnetic material is remanent upon exposure to a magnetic field and the matrix material has a surface comprising functional groups which promote disaggregation of the particles in the presence of a liquid phase.

Binding a target substance

The present invention relates to a method for isolating nucleic acids from a nucleic acid containing sample, and a kit for carrying out said method. More specifically, it relates to a novel method for extracting nucleic acids from a nucleic acid containing sample, using an anion exchange solid support, and allowing this solid phase with the nucleic acid bound thereto to react with a compound which is also capable of binding to said anion exchange solid support and which optionally provides additional charges at the surface of the anion exchange solid material, thereby preferably changing the surface charge density of the solid support and then releasing the nucleic acid from the solid support, eliminating the need for high salt and/or high pH elution buffers.

Nucleic acid extraction method

A process for the preparation of polymer magnetic particles, which comprises: providing polymer particles having a porous interior, and contacting the polymer particles with a magnetic fluid comprising a homogeneous dispersion of magnetic particles, whereby the magnetic particles are incorporated into the porous interior to produce polymer magnetic particles.

Preparation of polymer particles

A process for the preparation of polymer magnetic particles, which comprises: providing a water phase containing magnetic components homogeneously dispersed therein, wherein the water phase is contacted with or further contains a polymerisable metal-containing or organic monomer which is soluble in the water phase, and polymerizing the monomer in the presence of the magnetic components so as to form polymer magnetic particles in which the magnetic components are substantially uniformly distributed; wherein at least a part of the polymerizing step is carried out in a water-in-oil emulsion in which the water phase containing the magnetic components homogenously dispersed therein is present as a discontinuous phase in a continuous oil phase.

A process for isolating nucleic acid from a nucleic acid-containing sample, which comprises: (a) providing a chaotrope; (b) providing a nucleic acid binding solid phase capable of binding nucleic acid in the presence of the chaotrope; (c) providing a source of NH4+ or NH3; (d) contacting the sample with the nucleic acid binding solid phase in the presence of a liquid phase comprising the chaotrope and the NH4+ or NH3; and (e) optionally separating the solid phase with the nucleic acid bound thereto from the liquid phase.

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Haslum, Norway

Vidar Skagestad