Biomerieux, Inc.

Biomerieux Sa

Victoria Girard

Maud Arsac

John D Walsh

Jones M Hyman

Jean-Philippe Charrier

Yannick Charretier

Corinne Beaulieu

Parampal Deol

Pierre-Jean Cotte-Pattat

Christine Franceschi

Sylvie Pons

Valérie Monnin

Sonia Chatellier

Philippe Dufour

Elizabeth Miller

Amber Gates

Sandrine Mailler

John Walsh

A method for identifying by mass spectrometry an unknown microorganism subgroup among a set of reference subgroups, including a step of constructing one knowledgebase and one classifying model per associated subgroup on the basis of the acquisition of at least one set of learning spectra of microorganisms identified as belonging to the subgroups of a group and including: constructing an adjusting model allowing mass-to-charge offsets of the acquired spectra to be corrected on the basis of reference masses-to-charges that are common to the various subgroups; adjusting the masses-to-charges of all of the lists of peaks of the learning spectra and constructing one classifying model per subgroup and the associated knowledgebase on the basis of the adjusted learning spectra.

Method for identifying by mass spectrometry an unknown microorganism subgroup from a set of reference subgroups

A method for characterizing at least one microorganism from a sample includes identifying the at least one microorganism and determining the properties of typing, potential resistance to at least one antimicrobial, and virulence factor. The properties of typing, resistance to at least one antimicrobial, and virulence factor for the at least one microorganism are determined by implementing mass spectrometry using proteins, peptides and/or metabolites as markers of the properties of typing, resistance to at least one antimicrobial, and virulence factor.

Method for characterizing at least one microorganism by means of mass spectrometry

The present invention is directed to a method for inactivation and/or extraction of acid-fast bacteria (e.g.,or), the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain acid-fast bacteria and suspending the test sample in a container containing ethanol and beads; (b) bead beating and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria cells in the container; and (c) subsequently incubating the suspension for at least about 3 minutes at room temperature to inactivate any acid-fast bacteria contained in the test sample. In accordance with the present invention, the test sample can subsequently be pelleted by centrifugation, resuspended with formic acid, acetonitrile added, and subjected to mass spectrometry for characterization and/or identification of the acid-fast bacteria.

Methods for inactivation and extraction of acid-fast bacteria for characterization and/or identification using mass spectrometry

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Lyons, France

Victoria Girard