American Biogenetic Sciences, Inc.

Victoria A Ploplis

Paul E Gargan

Julian R Pleasants

The subject invention relates a method for the production of monoclonal antibodies. The method utilizes an immunized germfree animal. The invention also provides methods for the use of such monoclonal antibodies, and polyclonal antibodies derived from an immunized germfree animal, for in vitro and in vivo clinical diagnostics and therapeutics. Also, the subject invention provides a fibrin-specific monoclonal antibody.

Methods for treating a tumor containing fibrin using a fibrin specific monoclonal antibody and compositions used therein

Methods for treating a fibrin encapsulated tumor using a fibrin specific monoclonal antibody and compositions used therein

Methods for treating a fibrin encapsulated tumor using a fibrin specific

Methods and compositions for therapeutic uses of a fibrin-specific

A method of obtaining lymphocytes making a highly specific antibody by immunizing an antigen-free mouse and removing the blood, lymph nodes or spleen from the immunized mouse. Alternatively, a method of making highly specific poly clonal antibodies by immunizing an antigen-free mouse and removing serum from the mouse. Specific embodiments include multiple immunizations, immunizations with dissolved antigens, and immunizations with adjuvants.

Method comprising immunization of antigen-free mice

An in vitro immunoassay to detect and quantitate soluble crosslinked and non-crosslinked DesAABB fibrin polymers in a sample from a subject. The assay can be used to support a diagnosis of, to evaluate, and to monitor, in a mammalian subject, a thrombotic event, including, but not limited to, myocardial infarction, pulmonary embolism, stroke and deep vein thrombosis.

Immunoassay and kit for in vitro detection of soluble DesAABB fibrin

The subject invention relates a method for the production of monoclonal antibodies. The method utilizes an immunized antigen free animal. The invention also provides a method for the use of such monoclonal antibodies, and polyclonal antibodies derived from an immunized antigen free animal, for in vitro and in vivo clinical diagnostics and therapeutics. Also, the subject invention provides a fibrin-specific monoclonal antibody.

Method for production of antibodies utilizing an antigen-free animal

The subject invention relates to a fibrin-specific monoclonal antibody wherein said monoclonal antibody does not crossreact with: (a) fibrinogen, (b) plasmin derived fibrinogen degradation products and (c) plasmin derived fibrin degradation products.

Fibrin-specific monoclonal antibody

The subject invention relates to a fibrinogen-specific monoclonal antibody wherein said monoclonal antibody: (a) binds to the isolated A.alpha. chain of fibrinogen, (b) does not prolong the conversion time of fibrinogen to fibrin by thrombin, (c) binds to fibrin and (d) does not crossreact with plasmin derived fibrinogen degradation products. The subject invention also covers the use of such a monoclonal antibody in an immunoassay for the determination of plasma fibrinogen levels in vitro.

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Leuven, Belgium

Victoria A Ploplis