Centre National De La Recherche Scientifique

Rhone-poulenc Recherches

Victor Sanchez

Beatrice Biscans

Jean-Pierre Couderc

Jacques Pourrat

Ernest Casademont

Pierre Aimar

Marie-Andree Poulhes

J Pierre Lafaille

Jean Mahenc

Jean-Pierre Riba

Patrick Espenan

Jean-Pierre Lafaille

A method for allowing biological macromolecules contained in a solution to be separated by selective absorption comprising supplying with a solution each stage (3, 4) of a column containing a selective chromatographic resin specific to the macromolecules to be separated, so as to fluidize the beds of resin at each stage, each stage being provided at its base with a perforated distribution system (5) characterized by a percentage of open sections between 0.2% and 10%, a mean diameter of the said open sections greater than approximately 300 microns and between 2G.sub.m and 20G.sub.m, where G.sub.m is the mean granulometry of the resin.

Method for the separation of biological macromolecules by chromatography

Process and apparatus for separation of solid particles or

Electrophoretic fractionation apparatus well adopted for separating solutions containing diverse substances dissolved therein, e.g., such proteinaceous solutions as human plasma, is comprised of (i) a pair of opposed end plates, (ii) a pair of opposed electrodes, (iii) a pair of opposed frame members, (iv) at least two spaced membranes, said frame members, electrodes and membranes defining open electrode compartments between said end plates, and said frame members comprising inlet and outlet means for introducing and withdrawing an ionic solution into and from the central region of said electrode compartments, and (v) at least one fractionation plate disposed between said at least two membranes, and each said fractionation plate (v) comprising (1) at least one elongate slit extending through the thickness thereof and said slit defining, together with two of said spaced membranes, at least one solution fractionation chamber, (2) means for introducing solution to be fractionated into said at least one fractionation chamber, (3) means for withdrawing one fraction of fractionated solution from one end region of said fractionation chamber, and (4) means for withdrawing another fraction of fractionated solution from another end region of said fractionation chamber.

Electrophoretic fractionation apparatus

The fractionating apparatus of the invention for the separation of at least two dissolved groups of substances in a solution is of the type wherein an electric field is applied in fractionating chambers separated by semi-permeable membranes so as to generate different migrations by various groups of substances; each fractionating chamber is bounded by a peripheral frame 3 provided with a plurality of strips 23; the two semi-permeable membranes 1 and 2 of the chamber are applied on either side against the faces of said frame and against the faces of said strips; spacer compartments are advantageously provided on either side of the fractionating chambers, said spacer compartments containing an auxiliary solution capable of enhancing by high rate ion exchanges the differences in migrations of the substances to be separated.

Apparatus for electrophoretically fractionating a solution

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Ramonville Saint-Agne, France

Victor Sanchez