Dade Behring Inc.

Siemens Healthcare Diagnostics Gmbh

Victor P Chu

Zhu Teng

Tie Q Wei

William D Bedzyk

Timothy J Gorzynski

Ronald L Edwards

Steve P A Goss

Kelle M Garvey

Alan Robert Craig

Thomas John Pankratz

Sandra A Lewisch

James Floyd Pierson-Perry

Ronald A Edwards

Connie Mary Sanders

The present invention relates to immobilized procainamide analogs, as well as to a method of making immobilized procainamide analogs. These immobilized analogs are prepared by activating the carboxyl group on a substituted p-benzoic acid derivative toward nucleophilic attack; reacting the activated benzoic acid derivative with a polyamine to produce the benzoic acid derivative of Formula 3: and binding the benzoic acid derivative of Formula 3 to a latex polymer having functional groups that react with aliphatic amino groups. A method of conducting an immunoassay using the immobilized procainamide analog Formula 3 is described, comprising the steps of preparing a solution comprising said immobilized procainamide analog; adding a sample suspected of containing procainamide to said solution; adding an anti-procainamide antibody to said solution and observing the rate of increase in solution turbidity following antibody addition.

Synthesis and application of procainamide analogs for use in an immunoassay

Lidocaine analogs, latex particle-analog conjugates, and methods of making and using the same. The analogs have the formula: wherein The analogs are immobilized on latex particles and prepared for use in assays for lidocaine.

Lidocaine analogs and methods of making and using same

Calibrating an immunoassay by generating two reaction rate measuring curves, from samples having higher and lower relative levels of antigen, extrapolating a combination of the curves to cover sample concentrations known to contain an excess of antigen relative to an amount of capture reagent and combining the low end linear potion of the higher reaction rate measuring curve with the higher end portion of the extrapolated reaction rate measuring curve, thereby eliminating measuring inaccuracies otherwise arising from the hook effect. For antigen concentrations higher than the assay range, a high antigen signal utilizing the two rates avoids reporting false results.

Method for extending the range of an immunoassay

Calibration of an assay by converting an analyzer's normal signal response through an arithmetic function prior to performing the curve fit, thereby improving the accuracy of the curve fit in a region of greatest diagnostic interest.

Method for calibrating a chemical analyzer with improved accuracy at low signal levels

A method is provided for enhancing the stability and increasing the activity of polymer particle reagents by incubating a polymer particle which has been covalently bound to a compound of biological interest, such as a drug, with a modifying agent that will place a negative charge on the surface of the polymer particle and reduce residual functional groups. The modifying agent is preferably an anionic nucleophile selected from the group consisting of .beta.- mercaptoacetic acid and the thiosulfates.

Post synthesis chemical modification of particle reagents

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Hockessin, DE, United States of America

Victor P Chu