University of California

Vicki I Chin

Sangeeta N Bhatia

Michael J Sailor

Boyce E Collins

A nanoporous silicon support comprising a plurality of macropores is provided to function as a bioreactor for the maintenance of cells in culture in a differentiated state. Each cell or group of cells is grown in an individual macropore and is provided with nutrients by means such as perfusion of the nanoporous silicon support with fluid. The macropores may be between 0.2 and 200 microns and be coated with a substance that promotes cell adhesion. The support containing cells may be used to used to test compounds for biological activity, metabolism, toxicity, mutagenicity, carcinogenicity or to characterize novel or unknown comounds. The supports are sufficiently robust that they may be assembled into larger reactors to simulate organ function or be used for the production of biomolecules.

Method of screening compounds using a nanoporous silicon support containing macrowells for cells

A nanoporous silicon support comprising a plurality of macropores is provided to function as a bioreactor for the maintenance of cells in culture in a differentiated state. Each cell or group of cells is grown in an individual macropore and is provided with nutrients such as by perfusion of the nanoporous silicon support with fluid. The macropores may be between 0.2 and 200 microns and be coated with a substance that provides cell adhesion. The support containing cells may be used to used to test compounds for biological activity, metabolism, toxicity, mutagenicity, carcinogenicity or to characterize novel or unknown comounds. The support is sufficiently robust that it may be assembled into larger reactors to simulate organ function or be used for the production of biomolecules.

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La Jolla, CA, United States of America

Vicki I Chin