Centre National De La Recherche Scientifique

Université´de Rouen

Medicago Inc.

Stallergenes

Angany Genetics

Seita Groupe Altadis

Stallergenes S.a.

Angany Inc.

Véronique Gomord

Louis-Philippe Vezina

Patrice Lerouge

Muriel Bardor

Marc-André D'Aoust

Stéphanie Aquin

Loïc Faye

Christophe Rihouey

Thomas Paccalet

Loic Faye

Marc-Andre D'Aoust

Carole Burel

Ronald Van Ree

Erica Van Oort

Caroline Bonneau

Anne Catherine Fitchette

Virginie Catala

Marc-André D'AOUST

Estelle Marquet-Blouin

Stephanie Aquin

Anne-Catherine Fitchette

Marc-Andre D'aoust

Christophe Sourrouille

A method for producing a recombinant protein in a plant, in particular a tobacco plant, preferably, includes the following steps: a) culturing the plant aeroponically or hydroponically, preferably on mobile floats and under LED lighting; b) vacuum agroinfiltration of the plant obtained in a) by agrobacteria that include a DNA fragment coding for the recombinant protein; c) returning the plants to culturing after step b), under the same conditions as for step a); and d) extracting and purifying the recombinant protein from the aerial portions of the plants produced in step c).

Method for producing high-quality recombinant allergens in a plant

A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.

Synthesis of sialic acid in plants

A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5 Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.

A purified nucleic acid molecule comprising a nucleotide sequence coding for allergen Dac g5 having amino acid sequence SEQ ID NO. 2, a derivative or a fragment thereof.

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Growing community of inventors

Rouen, France

Véronique Gomord