Ajinomoto Co., Ltd.

Veronika Aleksandrovna Kotliarova

Sergey Vasilievich Smirnov

Natalia Nikolaevna Samsonova

Natalia Yurievna Rushkevich

Sakayu Shimizu

Jun Ogawa

Yury Ivanovich Kozlov

Tomohiro Kodera

Makoto Hibi

Mikhail Markovich Gusyatiner

Masakazu Sugiyama

Yulia Georgievna Rostova

Tatyana Abramovna Yampolskaya

Leonid Romanovich Ptitsyn

Vitaly Grigorievich Paraskevov, Legal Representative

Irina Borisovna Altman

Sergey Vasil'evich Smirnov

Anna Eugenievna Novikova

Shunichi Suzuki

Yuki Imabayashi

Hidemi Fujii

Tatyana Aleksandrovna Bachina

Olga Sergeevna Beznoschenko

Olga Nikolaevna Mokhova

The present invention provides a method for producing glycine or a salt thereof by fermentation of a bacterium which has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity. The bacterium can be, for example, a coryneform bacterium or a bacterium belonging to the family Enterobacteriaceae.

Method of producing glycine by fermentation

The present invention provides a coryneform bacterium having an ability to produce a heterologous fusion protein by secretory production, which has been modified to express a genetic construct for secretory production of the heterologous fusion protein encoding at least a heterologous fusion protein comprising an extein and an intein having an activity of acyl rearrangement. The method for producing proteins modified at the C-terminus is also provided.

Coryneform bacterium and method for producing heterologous fusion proteins

A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.

Bacterium producing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity and a method for manufacturing the product

A highly active L-isoleucine dioxygenase fromis provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

Method for producing 4-hydroxy-L-isoleucine

L-arginine, citrulline and pyrimidine derivatives including orotic acid, uridine, uridine 5'-monophosphate (UMP), cytidine and cytidine 5&#x2032;-monophosphate (CMP) are produced using a bacterium belonging to the genusharboring a mutant carbamoylphosphate synthetase in which the amino acid sequence corresponding to positions from 947 to 951 in a wild type carbamoylphosphate synthetase is replaced with any one of amino acid sequences of SEQ ID NOS: 1 to 9, and feedback inhibition by uridine 5&#x2032;-monophosphate in the bacterium is desensitized.

Mutant carbamoylphosphate synthetase and method for producing compounds derived from carbamoylphosphate

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Moscow, Russia

Veronika Aleksandrovna Kotliarova