Cargill Inc.

Bioamber Inc.

Vernon L McIntosh, Jr

Brian Jeffrey Rush

Gregory Michael Poynter

Arlene M Fosmer

Kevin T Watts

Thomas W McMullin

Beth M Mastel

Jeanette M Huryta

Kenneth R Finley

Keith M Brady

Yeast cells having a reductive TCA pathway from pyruvate or phosphoenolpyruvate to succinate, and which include at least one exogenous gene overexpressing an enzyme in that pathway, further contain an exogenous transhydrogenase gene.

Yeast cells having reductive TCA pathway from pyruvate to succinate and overexpressing an exogenous NAD(P+) transhydrogenase enzyme

The present application provides genetically modified yeast cell comprising an active succinate fermentation pathway, as well as methods of using these cells to produce succinate.

Compositions and methods for succinate production

Yeast cells having reductive TCA pathway from pyruvate to succinate and overexpressing an exogenous NAD(P)+ transhydrogenase enzyme

Recombinant yeast cells contain a reductive TCA pathway from phosphoenolpyruvate or pyruvate to succinate. At least one metabolic step in the pathway includes a reaction of NADPH to produce NADP+. The yeast cell contains at least one exogenous NADPH-dependent gene in the pathway from phosphoenolpyruvate or pyruvate to succinate, preferably an NADPH-dependent malate dehydrogensase or fumarate reducase gene (or both).

Yeast cells having NADP(H)-dependent reductive TCA pathway from pyruvate to succinate

Growing community of inventors

Minneapolis, MN, United States of America

Vernon L McIntosh, Jr