Genzyme Corporation

Integrated Genetics, Inc.

Exemplar Corporation

Vermuri B Reddy

Nancy Hsiung

Anton K Beck

Edward G Bernstine

Cha-Mer Wei

William R Dackowski

Jeffrey F Lemontt

Edward S Cole

Edward G Berstine

David Tai-Yui Lau

Richard D Purcell, Jr

Richard Douglas

Edward George Bemstine

A rapid, sensitive, and quantitative method and assay system using transgenic animal cells and adult or embryonic transgenic animals to rapidly screen compounds for mutagenic or teratogenic activity. Each cell from a cell culture, a differentiated tissue from an animal or an animal embryo contains a target gene and a reporter gene, both including an animal promoter/enhancer, a coding sequence and a transcription termination signal, so that they are capable of expression in animal cells. The product of the target gene is capable of regulating expression of the reporter gene through interaction with a regulatory sequence in the reporter gene. In the preferred embodiment, the target gene lacI (lac repressor) is coupled to the reporter gene lacZ encoding beta-galactosidase, which is easily detectable by cytochemical or histochemical procedures. When the animal cell is exposed to a compound causing a mutation either altering expression of the target gene or altering the operator of the reporter gene, the reporter gene is expressed.

Rapid screening mutagenesis and teratogenesis assay

Biologically active heterodimeric human fertility hormones composed of two different subunits, each subunit being synthesized in the same cell transformed by at least one cell expression vector having heterologous DNA encoding each subunit with each subunit being controlled by a separate promoter. Preferred human fertility hormones include hCG, hLH and hFSH.

Nucleic acids encoding and recombinant production of the .beta. subunit

Recombinant heterodimeric human fertility hormones, and methods, cells,

The correct sequence is disclosed for the beta subunit of human follicle stimulating hormone which, when present in a recombinant heterodimeric human fertility hormone allows the production of biologically active hormone. DNA coding for such beta subunit of human FSH may thus be prepared as well as expression vectors containing such DNA and cells transfected therewith.

DNA encoding the beta subunit of human follide stimulating hormone and

A replicable expression vector capable, in a transformed host mammallian cell, of expressing a DNA sequence encoding active human TPA which, when expressed in the host cell, yields active TPA which lacks one or more of the TPA-bound N-linked carbohydrate moieties of naturally occurring human TPA.

Human uterine tissue plasminogen activator produced by recombinant DNA

Biologically active heteropolymeric protein composed of a plurality of subunits, each subunit being synthesized by a cell having an expression vector heterologous DNA encoding the subunit.

Heteropolymeric protein

Growing community of inventors

Westboro, MA, United States of America

Vermuri B Reddy