Ptt Global Chemical Public Company Limited

Verawat Champreda

Navadol Laosiripojana

Phatthanon Prasitchoke

Wikanda Techanan

Natthawut Poomsila

Aphisit Poonsrisawat

Napong Ketsub

Nopparat Suriyachai

Jantima Arnthong

Surisa Suwannarangsee

Naphatsaya Denchokpraguy

Chatuphon Siamphan

Mahattanee Phinyo

Navado Laosiripojana

The present invention relates to a novel mutant strainE14-292 and a genetic modification process of said strain, wherein the mutant strain according to this invention can produce cellulase and xylanase more than the BCC199 (wild type). Moreover, the obtained enzymes can be used to digest the pretreatment bagasse to further produce sugars effectively.

Mutant strainfor producing cellulase and xylanase and preparation method thereof

This invention aims to develop a process for fractionation of lignocellulosic biomass by contacting lignocellulosic biomass with treatment liquid in close system at temperature not over 200&#xb0; C. and pressure of 5 to 20 bars, wherein said treatment liquid comprises water-miscible part that comprising formic acid, alcohol, and water and water-immiscible part that comprising alkyl acetate. The lignocellulosic biomass may be selected from rice straw, bagasse, corncobs, corn stem, palm waste, grass, pineapple shell, bamboo, or mixture thereof.

Process for fractionation of lignocellulosic biomass

The present invention relates to a pretreatment process of lignocellulosic biomass, comprising contacting lignocellulosic biomass with pretreatment solution in a temperature ranging from an ambient temperature to 100&#xb0; C. and a pressure of at least 20 bars, wherein the pretreatment solution comprising water, water-miscible organic solvent and alkali having a concentration of 5 to 15% w/v if the alkali is solid or 5 to 15% v/v if the alkali is liquid. Said pretreatment process operates in mild condition or at low temperature to be specified with the removing of lignin but not destroying cellulose and increasing desired sugar yield.

Pretreatment process of lignocellulosic biomass

Growing community of inventors

Bangkok, Thailand

Verawat Champreda