Qiagen Gmbh

Preanalytix Gmbh

Vera Holländer

Thomas Rothmann

Ralf Wyrich

Uwe Oelmüller

Markus Müller

Gabriele Christoffel

Christian Lenz

Martin Schlumpberger

Karin Schulte

Peter Porschewski

Margit Hiesinger

Andre Schrader

Stefanie Schröer

Daniel GRÖLZ

Ralph Wyrich

The present invention pertains to a method for isolating a target nucleic acid including small target nucleic acids from a sample, said method comprising at least the following steps a) binding at least a portion of the target nucleic acid including small target nucleic acids to a nucleic acid binding solid phase comprised in a column by passing the sample through said column, b) performing an enzymatic and/or chemical treatment on the nucleic acid binding solid phase while the target nucleic acid is bound to said solid phase, c) collecting at least a portion of the small target nucleic acids released from the solid phase during said treatment of step b) as flow-through, d) contacting said flow-through which comprises small target nucleic acids mixed with a recovery solution with a nucleic acid binding solid phase for binding the contained small target nucleic acids to said nucleic acid binding solid phase, e) optionally performing an elution. The present invention results in a considerable increase in the yield of small target nucleic acids in the isolated target nucleic acid because it allows to efficiently capture and recover small target nucleic acids.

Method for isolating a target nucleic acid including small target nucleic acids with high yield

The present invention refers to a device, comprising a hollow body having at least one open end comprising at least one solid matrix binding, adsorbing, absorbing, chelating or retaining compounds which are not desired in a sample and preferably at least one barrier which is non-permeable for liquids and solids under ambience conditions, however, becomes liquid-permeable by applying an external force to the barrier, the use of such a device for isolating or purifying a biomolecule from a sample, a method for preparation of the device and a method for isolation or purification of any biomolecule using said device.

Device for isolation and/or purification of biomolecules

The present invention provides a method for isolating RNA including small RNA having a size of 200 nt or less from a sample, comprising the following steps: a) providing a composition comprising RNA and a chaotropic agent; b) adding alcohol; c) incubating the mixture for at least 2 min; d) adding additional alcohol to the mixture to adjust the overall alcohol concentration in the mixture to &#x2265;50%; e) binding RNA contained in the mixture to a nucleic acid binding solid phase; f) optionally washing the bound RNA; g) optionally eluting RNA from the solid phase. Due to the step-wise addition of alcohol, the overall RNA yield and the yield of small RNA is improved.

Method for isolating RNA including small RNA with high yield

The present invention pertains to a method for isolating RNA, including small RNA from a RNA and DNA containing sample, wherein the sample is lysed and the optionally further processed lysate is incubated with a DNase to degrade DNA prior to purifying the RNA from the optionally further processed lysate. It was found that performing the DNase digest prior to isolating the RNA from the lysate has considerable advantages.

Method for isolating RNA from a RNA and DNA containing sample

The present invention refers to a device, comprising a hollow body having at least one open end and at least one barrier inside or at the end of the hollow body, which is non-permeable for liquids and solids under ambience conditions, however, becomes liquid-permeable by applying an external force like pressure, drag force or driving power to said barrier, the use of such a device for collection, storage, treatment or isolation of a biomolecule or biomolecules containing samples, a method for preparation of said device and a method for isolation or purification of any biomolecules using said device.

Storage, collection or isolation device

The present invention relates to a method of isolating/extracting in parallel various biomolecules, in particular nucleic acids and proteins, from the same fixed biological samples, to the quantification and analysis of the biomolecules isolated by the method of the invention, and to a kit for isolating/extracting in parallel various biomolecules from a fixed sample, to the use of said kit for diagnosing, prognosing, deciding the therapy of and monitoring the therapy of a disease.

Parallel extraction of different biomolecules from formalin-fixed tissue

The present invention relates to a process for the parallel isolation and/or purification of RNA and DNA from the same fixed biological sample, the quantification and analysis of the nucleic acids isolated by the process according to the invention, to a kit for the parallel isolation and/or purification of RNA and DNA from a fixed sample and to the use of this kit for the diagnosis, prognosis, decision with respect to therapy and/or the monitoring of the therapy of a disease.

Process for parallel isolation and/or purification of RNA and DNA

The invention relates to a method for treating a biological sample, comprising the following method steps: i) preparation of a biological sample and ii) bringing the biological sample into contact with a composition, comprising: (1) 1 to 100 wt. % of at least one polyol and (2) 0 to 99 wt. % of at least one additive, wherein the total amount of components (1) and (2) is 100 wt. %. The invention further relates to biological samples obtained by said method, a method for analysis of a treated biological sample, devices for treating a biological sample, use of said devices, various kits and use of a composition.

Method for treating a biological sample with a composition containing at least one polyol and excluding water

The present invention relates to a method of stabilizing a biological sample, having the steps of preparation of a biological sample, and of contacting the biological sample with a composition, having a substance according to the following structural formula: in which R1 is a hydrogen residue or a methyl residue, R2 and R3 are identical or different hydrocarbon residues with a length of the carbon chain of 1-20, and R4 is an oxygen, sulfur or selenium residue (FIG.).

Method for stabilising a biological sample

The present invention relates to new compositions for isolating and/or stabilizing nucleic acids in materials of biological origin. The compositions comprise as an essential ingredient a cationic compound of general formulaYRRRRXwherein

Compositions for the isolation and/or stabilization of nucleic acids in biological materials

The present invention relates to new compositions for isolating and/or stabilising nucleic acids in materials of biological origin. The compositions comprise as an essential ingredient a cationic compound of general formulaYRRRRXwherein

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Unna, Germany

Vera Holländer