University of Utah Research Foundation

Medical Research Council

Venkatraman Ramakrishnan

Brian Thomas Wimberly

Andrew Philip Carter

Ditlev Egeskov Brodersen

William Melvon Clemons, Jr

The invention provides an X-ray crystal structure of the 30S ribosome, obtained from30S subunit, having a tetragonal space group P422 with unit cell dimensions of a=401.4&#xb1;4.0 &#x212b;, b=401.4&#xb1;4.0 &#x212b;, c=175.9&#xb1;5.0 &#x212b;. An advantageous feature of the structure is that it diffracts beyond 3 &#x212b; resolution. The invention also provides a crystal of 30S having the three dimensional atomic coordinates of the 30S ribosome, the coordinates being provided in Tables 1A and 1B. The data may be used for the rational design and modelling of inhibitors for the 30S ribosome, which have potential use as antibiotics.

Crystal structure of the 30S ribosome and its use

The invention provides high resolution X-ray crystal structures of the 30S ribosome, obtained from30S subunit, having a tetragonal space group P422 to which are bound an antibiotic selected from the group paromomycin, streptomycin, spectinomycin, tetracycline, pactamycin and hygromycin B. An advantageous feature of the structure is that it diffracts at about 3 &#x212b; resolution. The invention also provides a crystal of 30S having the three dimensional atomic coordinates of the 30S ribosome, the coordinates being provided in any one of tables 1 to 4. The data may be used for the rational design and modelling of inhibitors for the 30S ribosome, which have potential use as antibiotics.

Crystal structure of antibiotics bound to the 30S ribosome and its use

Crystal structure of the 30s ribosome

The present invention is broadly directed to methods of screening ribosomal protein L11/GTPase activating region (GAR) RNA-modulating compounds by using information from the high-resolution structure of the L11/GAR complex. The methods are useful in identifying compounds useful for anti-bacterial treatments.

Growing community of inventors

Cambridge, United Kingdom

Venkatraman Ramakrishnan