Yield10 Bioscience, Inc.

Venkatesh Bollina

Frank Anthony Skraly

Kristi D Snell

Meghna Malik

Oliver P Peoples

Jihong Tang

Madana M R Ambavaram

Nirmala Sharma

Claire Burkitt

Kieran Ryan

Yuanyuan Ji

A modified plant is provided. The modified plant includes a polynucleotide including a promoter operably linked to a coding sequence. The coding sequence encodes a transcription factor that has at least 30% sequence identity to one or more of SEQ ID NOs: 27, 204, 10, 19, 1-9, 11-18, 20-26, 28-203, 205, 237, or 242-310. The promoter is non-cognate with respect to the coding sequence. Also provided is a method for producing a modified plant having increased seed yield and/or oil content in comparison to a reference plant by transformation. Also provided is a method for producing a modified plant having increased seed yield and/or oil content in comparison to a reference plant by editing.

Modified plants comprising a polynucleotide comprising a non-cognate promoter operably linked to a coding sequence that encodes a transcription factor

A genetically engineered land plant that expresses a protein that has homology to a plant invertase inhibitor and/or a pectin methylesterase inhibitor and that increases seed yield with increased expression ('an ISY protein') is disclosed. The plant comprises a modified gene for the ISY protein. The ISY protein comprises one or more of an ISY protein ofcomprising SEQ ID NO: 2 or a fragment,homolog, or ortholog thereof. The modified gene comprises a promoter and a nucleic acid sequence encoding the ISY protein. The promoter is non-cognate with respect to the nucleic acid sequence encoding the ISY protein. The modified gene is configured such that transcription of the nucleic acid sequence is initiated from the promoter and results in expression of the ISY protein. A genetically engineered land plant that expresses an RNA that increases seed yield with increased expression also is disclosed.

Genetically engineered land plants that express an increased seed yield protein and/or an increased seed yield RNA

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Saskatoon, Canada

Venkatesh Bollina