Wisconsin Alumni Research Foundation

Cellular Dynamics International, Inc.

Fujifilm Cellular Dynamics, Inc.

Veit Bergendahl

James A Thomson

Richard R Burgess

Nathaniel Beardsley

Christine Daigh

Terrance M Arthur

Larry C Anthony

Megan Fitzgerald

Bradley C Pietz

The invention concerns methods for automated culture of embryonic stem cells (ESCs) such as human ESCs. In some aspects, methods of the invention employ optimized culture media and limited proteolytic treatment of cells to separate cell clusters for expansion. Automated systems for passage and expansion of ESCs are also provided.

Automated method and apparatus for embryonic stem cell culture

While culture medium and systems have been described that permit the culture and proliferation of human embryonic stem cells in feeder free and animal product free conditions, these conditions will not readily support cloning of an embryonic stem cell culture meaning, at least here, the initiation of a sub-culture using one or a very few originating cells. It has been found here that a class of small molecules that are inhibitors of kinase enzymes will increase the efficiency of cloning of stem cell cultures sufficiently to make such cloning practical in the defined medium and in other media as well.

Clonal culture of human pluripotent stem cells

The invention provides a method to identify inhibitors of the formation of holoenzyme from core RNA polymerase and sigma.

Sigma binding region of RNA polymerase and uses thereof

Growing community of inventors

Madison, WI, United States of America

Veit Bergendahl