Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-La Roche Ag

Veeraiah Bodepudi

Stephen Gordon Will

Amar Gupta

David H Gelfand

Thomas W Myers

Fred Lawrence Reichert

Nancy J Schoenbrunner

Ellen H Fiss

Rachel Shahinian

Keith Bauer

Alison Tsan

John Niemiec

David Harrow Gelfand

Amar P Gupta

Stephen Will

Keith A Bauer

The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having at least one selective nucleotide complementary to only one variant of the target sequence and incorporating at least one 'G-clamp' nucleotide.

Use of G-clamp for improved allele-specific PCR

The present invention provides reagents for use in the amplification of nucleic acids. Amplification carried out using oligonucleotides containing modified nucleotides can result in less non-specific amplification compared to amplification carried out using unmodified oligonucleotides.

Methods and reagents for reducing non-specific amplification

Alkyl amines act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting alkyl amines to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization.

Alkyl amines improve detection of components of formaldehyde-fixed biological samples

The present invention provides methods and reagents for use in the amplification of nucleic acids. Amplification carried out using oligonucleotides containing modified nucleotides can result in less non-specific amplification compared to amplification carried out using unmodified oligonucleotides.

The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2'-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.

2'-terminator nucleotide-related methods and systems

The invention provides compositions that comprise nucleotides and/or nucleosides having blocking groups at 2'-positions of sugar moieties. Methods of synthesizing these nucleic acids are also provided.

Synthesis and compositions of 2'-terminator nucleotides

The invention provides methods of producing blocked oligonucleotides that include 2'-terminator nucleotides. These blocked oligonucleotides are used primers and probes in a variety of nucleic acid technologies. Related kits are also provided.

Synthesis and compositions of nucleic acids comprising 2'-terminator nucleotides

2'-Terminator nucleotide-related methods and systems

The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2'-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2&#x2032;-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and/or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.

2'-terminator related pyrophosphorolysis activated polymerization

The invention relates to detectable labels useful for detection of nucleotide sequences. Specifically, the invention relates to labeled-imidazole-PEG compounds, such as nucleosides, nucleotides, and nucleic acids incorporating such compounds, and methods utilizing such compounds. The invention further relates to kits comprising labeled imidazole-PEG compounds.

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San Ramon, CA, United States of America

Veeraiah Bodepudi