Philip Morris Inc.

Vedpal S Malik

Herbert Y Nakatani

Bernard A Semp

Daniel M Teng

Hernan G Bravo

Samir Z Wahab

Nicotine acid sequences encoding a tobacco protein involved in nicotine synthesis are described. These sequences, when inserted in to sense or anti-sense orientation, affect nicotine synthesis in transgenic tobacco plants.

Purified tobacco protein involved in nicotine synthesis, DNA encoding,

Protein involved in nicotine synthesis, DNA encoding, and use of sense

There is provided highly purified tobacco putrescine N-methyltransferase, a process for its purification, and production of PMT DNA sequence. The purification process includes the step of applying a tobacco root extract to an anion exchange medium and specifically eluting putrescine N-methyltransferase with an elution buffer comprising putrescine.

Method of purifying putrescine n-methyltransferase from tobacco plant

Method of purifying putrescine N-methyltransferase from tobacco plant

High temperature processes and thermophilic organisms for use in those processes for reducing the levels of certain nitrogen-containing compounds in tobacco materials. Tobacco materials are contacted with at least one thermophilic organism characterized by an anaerobic, dissimilatory, metabolic pathway for denitrification of tobacco materials under anaerobic and high temperature conditions that promote such metabolism. Tobacco materials treated in accordance with these high temperature processes and thermophilic organisms, when incorporated into a smoking product, deliver a significantly reduced amount of oxide of nitrogen in smoke. Moreover, such tobacco materials also afford the product of other tobacco products having lower amounts of nitrates and other nitrogen-containing compounds.

Thermophilic denitrification of tobacco

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Richmond, VA, United States of America

Vedpal S Malik