Cargill Inc.

Cargill Dow Polymers LLC

Nature Work, Llp

Vassily Hatzimanikatis

Jeffrey John Kolstad

Aharon Eyal

Vineet Rajgarhia

Ting Liu Carlson

John N Starr

Stacey Olson

The invention provides methods and materials related to the production of lactic acid. Specifically, the invention provides methods for producing lactic acid using a crabtree-negative yeast, such as of theandgenera, which have been transformed with a lactate dehydrogenase gene.

Methods for the synthesis of lactic acid using crabtree-negative yeast transformed with the lactate dehydrogenase gene

Crabtree negative organisms such as Kluyveromyces, Pichia, Hansenula and Candida, are used to make selected organic products such as lactic acid. The organisms are cultured in a first culture medium that includes glucose, under conditions that promote cellular respiration. The organisms are then cultured under a second set of conditions that promote production of the selected organic product. The organisms preferably contain an exogenous lactate dehydrogenase gene.

Production of lactate using crabtree negative organisms in varying culture conditions

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Minneapolis, MN, United States of America

Vassily Hatzimanikatis