E.i. Dupont De Nemours and Company

Butamax Advanced Biofuels LLC

Butamax (tm) Advanced Biofuels LLC

Genencor International, Inc.

Other

Genex Corporation

Nagarajan, Vasantha

Banner, Carl D.b.

Rhodes, Craig S.

Vasantha Nagarajan

Andrew C Eliot

Gail K Donaldson

Michael G Bramucci

Dennis Flint

Lori Ann Maggio-Hall

Charles Edwin Nakamura

Stuart M Thomas

Mario W Chen

Lisa Anne Laffend

Qiong Cheng

Jean-Francois Tomb

Natalia Sedkova

Manjari Singh

Helene Marie Albertson-Kane

Tina K Van Dyk

Kristy N Kostichka

David Richard Corbin

Pierre E Rouviere

Robert Ray Burch

Wonchul Suh

Ranjan Patnaik

Lixuan Lisa Huang

Katharine Janet Gibson

Robert A Larossa

Edward S Miller, Jr

Patricia C Brzostowicz

Carol M McCutchen

Robert R Dorsch

Vidya Pai

Dominic A Dragotta

Mark Scott Payne

Perry G Caimi

Gregory Marshall Whited

Stephen K Picataggio

Rick Weizhang Ye

Luan Tao

Donald Eugene Trimbur

Michael Charles Grady

Sharon Loretta Haynie

Arthur Leo Kruckeberg

Siqun Wang

Ramesh V Nair

Pamela L Sharpe

Anthony Arthur Gatenby

Maria R Diaz-Torres

Yuying Zhang

Ritu Bhalla

Lisa L Huang

Mehmedalija Jahic

Dana R Smulski

Bhaskar Bhadra

Amy Kuang-Hua Hsu

Kristy Nan-Shan Kostichka

Richard D La Reau

Leslie B Tang

Carl D Banner

Craig S Rhodes

Helene M A Kane

Richard D Lareau

Stephen Kenneth Picataggio

Carl Db Banner

Edward S Miller Jr

The invention relates to the fields of industrial microbiology and alcohol production. The invention also relates to the development of a microorganism capable of producing fermentation products via an engineered pathway, and uses of the microorganism. The invention also relates to the methods to improve cell viability and productivity and the use of recycling and acid washing to increase the yield of fermentation products.

Fermentative production of alcohols

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

Fermentive production of four carbon alcohols

Method for the production of isobutanol by recombinant yeast

Methods for the fermentive production of four carbon alcohols is provided. Specifically, butanol, preferably 1-butanol is produced by the fermentive growth of a recombinant bacterium expressing a 1-butanol biosynthetic pathway.

Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. Yeast host cells provided herein comprise reduced pyruvate decarboxylase activity and modified adenylate cyclase activity. In embodiments, yeast host cells provided herein comprise resistance to butanol and increased biomass production.

Butanol tolerance in microorganisms

Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein.

Recombinant host cell comprising a diol dehydratase

Production of isobutanol by a microorganism with enhanced acetolactate synthase activity

Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein. Specifically disclosed herein are the use of coenzyme B-independent butanediol dehydratases that catalyzes the substrate to product conversion of 2,3-butanediol to 2-butanone in the process of producing 2-butanol and 2-butanone.

Growing community of inventors

Wilmington, DE, United States of America

Vasantha Nagarajan