Albert Einstein College of Medicine

Other

Harvard College

Vasan Sambandamurthy

William R Jacobs, Jr

Stoyan S Bardarov

Tsungda Hsu

Svetoslav Bardarov, Legal Representative

Barry R Bloom

Mary K Hondalus

Sheldon Morris

Samantha Sampson

Methods of treating a mammal that is deficient in CD4and/or CD8lymphocytes are provided. The methods comprise inoculating the mammal with an attenuated mycobacterium in thecomplex. In these methods, the mycobacterium comprises two deletions, wherein a virulent mycobacterium in thecomplex having either deletion exhibits attenuated virulence. Use of these mycobacteria for the manufacture of a medicament for the treatment of mammals deficient in CD4and/or CD8lymphocytes is also provided.

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Non-naturally occurring mycobacteria in thecomplex are provided. These mycobacteria have a deletion of an RD1 region or a region controlling production of a vitamin, and exhibit attenuated virulence in a mammal when compared to the mycobacteria without the deletion. Also provided are non-naturally occurring mycobacteria that have a deletion of a region controlling production of lysine, and mycobacteria comprising two attenuating deletions. Vaccines comprising these mycobacteria are also provided, as are methods of protecting mammals from virulent mycobacteria using the vaccines. Also provided are methods of preparing these vaccines which include the step of deleting an RD1 region or a region controlling production of a vitamin or the amino acids leucine and lysine from a mycobacterium in thecomplex. Embodiments of these mycobacteria, vaccines and methods, encompassing mycobacteria comprising a leucine auxotrophy and a pantothenate auxotrophy, are also provided.

Non-naturally occurring mycobacteria in thecomplex are provided. These mycobacteria have a deletion of an RD1 region or a region controlling production of a vitamin, and exhibit attenuated virulence in a mammal when compared to the mycobacteria without the deletion. Also provided are non-naturally occurring mycobacteria that have a deletion of a region controlling production of lysine, and mycobacteria comprising two attenuating deletions. Vaccines comprising these mycobacteria are also provided, as are methods of protecting mammals from virulent mycobacteria using the vaccines. Also provided are methods of preparing these vaccines which include the step of deleting an RD1 region or a region controlling production of a vitamin from a mycobacterium in thecomplex.

Growing community of inventors

Bronx, NY, United States of America

Vasan Sambandamurthy