Auburn University

Valery A Petrenko

Vitaly J Vodyanoy

Tatiana I Samoylova

Nancy R Cox

Bryan A Chin

Zhongyang Cheng

Alexandre M Samoylov

Henry J Baker

Ludmila Globa

James Barbaree

James W Gillespie

Howard C Wikle

Anna M Cochran

Tung-Shi Huang

Nancy E Morrison

Deepa Bedi

Olusegun A Fagbohun

Jennifer Cannon Sykora

Disclosed are targeted particles comprising or consisting of a plurality of landscape phage fusion proteins complexed with heterologous nucleic acid, the landscape phage fusion proteins displaying a heterologous peptide and the targeted particle binding specifically to a target site. The particles may be utilized in methods for modulating expression of genes in target cells.

Targeted particles comprising landscape phage fusion proteins and heterologous nucleic acid

Disclosed are methods, compositions, zona pellucida binding peptides and polypeptides, and expression vectors for use in species-specific immunocontraception of animals, which include landscape bacteriophage. The disclosed compositions may include immunogenic compositions or vaccines.

Phage constructs, sequences and antigenic compositions for immunocontraception of animals

A targeted drug delivery nanocarrier and a method of forming the same is disclosed herein. The targeted drug delivery nanocarrier includes a plurality of amphipathic molecules forming a carrier particle having a plurality of drug molecules contained therein. A targeted landscape phage protein assembly is complexed to the carrier particle preferably using the unique method disclosed herein. The targeted landscape phage protein assembly displays a binding peptide that is selected to specifically and selectively bind to a target site. The method for forming targeted drug delivery nanocarriers includes the steps of obtaining a plurality of bacteriophage displaying a binding peptide for a desired target site, treating the bacteriophage with a denaturing agent, mixing the treated bacteriophage with a plurality of carrier particles and purifying the mixture to obtain a plurality of targeted drug delivery nanocarriers.

Drug delivery nanocarriers targeted by landscape phage

A magnetostrictive ligand sensor device (MLSD), system and method are provided having at least one magnetostrictive particle (MSP) to which is bound at least one binding element. The MSP may be dispersed in a sample containing a target ligand such that the target ligand binds thereto. A driver is also provided to emit a varying magnetic field such that the MSP produces a resonance response detectable by a measurement device configured to receive and detect changes in the resonance response that are due to alterations of the binding element. The MLSD and assays find use in identifying and quantitating ligands as well as chemicals and environmental conditions in a sample or patient.

Magnetostrictive ligand sensor

Methods and compositions for identifying and characterizing the affinity of one or more ligands of a peptide are provided. In particular, a 'stripped phage ligand sensor device' (SPLSD) is provided comprising a sensor coupled to a binding element of interest. Binding elements of the invention comprise phage which in most embodiments express a peptide of interest on the phage surface. Assays using the SPLSD allow detection and quantitation of ligands. Also provided are improved methods for forming monolayers using phage. In particular, methods for the formation of monolayers using 'stripped phage' are provided. Further provided are monolayers and Langmuir-Blodgett films formed by the methods of the invention as well as substrates having deposited thereon the films of the invention. The monolayers, films and substrates of the invention are useful as components of biosensors and/or chemosensors.

Method of forming monolayers of phage-derived products and used thereof

Compositions for use in characterization, diagnosis, prognosis, and therapy of cancer cells are provided. The compositions comprise peptides and variants thereof which were isolated based on their ability to selectively bind glioma cells.

Peptides for recognition and targeting of GLIAL cell tumors

Growing community of inventors

Auburn, AL, United States of America

Valery A Petrenko