Consejo Superior De Investigaciones Cientificas

Mecwins, S.a.

Valerio Pini

Francisco Javier Tamayo De Miguel

Priscila Monteiro Kosaka

Daniel Ramos Vega

Jose Jaime Ruz Martinez

Andreas Thon

Montserrat Calleja Gómez

Montserrat Calleja Goméz

Antonio Salvador-Matar Renteria

Virginia Cebrián Hernando

Carlos García Aguado

Maria Ujue Gonzalez Sagardoy

Jesús Oscar Ahumada Heredero

Oscar Malvar Vidal

Mario Encinar Del Pozo

Sheila Gonzalez Castilla

Carlos GARCÍA Aguado

Jesús Oscar Ahumada Herrero

Virginia CEBRIÁN Hernando

The present invention relates to a system for biodetection applications comprising two basic elements, a substrate with a functionalized surface and a nanoparticle, the system being capable of enhancing the plasmonic effect of the nanoparticle. The invention also relates to a biosensor incorporating such system, in addition to the method for detecting and quantifying a target analyte selected in a sample using such system. Finally, the invention relates to a device which can detect the enhanced optoplasmonic effect of the nanoparticles by means of the system of the invention or by combining the detection of such optoplasmonic effect with the analysis of the changes in the mechanical characteristics in the substrate.

System for biodetection applications

Biosensing platform for simultaneous, multiplexed, high throughput and ultra-sensitive optical detection of biomarkers labelled with plasmonic nanoparticles, the platform being provided with a biosensor, a broadband and continuous spectrum illumination source, an optical detector for simultaneously capturing spatially resolved and spectrally resolved the scattering signal of each individual nanoparticle, an autofocus system and an optical system adapted to collect the scattered signal of the biosensor's surface onto the optical detector, the platform being provided with translation means for the optical system and/or the biosensor, such that the optical system and the biosensor can be displaced relative to each other in the three dimensions, and wherein the processing means are adapted to: i) simultaneously capture spatially and spectrally resolved scattering signals from each nanoparticle individually, and ii) to analyze these signals simultaneously with the capture process.

Biosensor platform and method for the simultaneous, multiplexed, ultra-sensitive and high throughput optical detection of biomarkers

A method for optically detecting biomarkers in a biosensor is disclosed, wherein the optical detection obtains spatially and spectrally resolved optical signals from a sample on a biosensor, and one or more of these spatially and spectrally resolved optical signals can be analyzed in parallel with image acquisition. The image analysis comprises reading data of the acquired images, correcting them to reduce inhomogeneities and noise, localizing particles in the images, characterizing each particle individually to obtain its position and characterization parameters, and classifying the particles based on their characterization parameters. Using the number of particles per class for all the acquired images of the sample, a statistical value is calculated per sample and each statistical value is correlated with an indication of the presence of a biomarker in the sample.

Method for optically detecting biomarkers

The invention relates to a spectrophotometer, especially a spectrophotometer that can carry out simultaneous analysis at different points on the same sample (), with a high spatial resolution and without requiring a mechanical system for physical scanning along the sample. This is obtained by the provision of means for processing the light received by the photodetectors (), said processing means having a correlation wherein each of the photodetectors () corresponds to a spatial point on the sample (). In the case of dark field applications, the present invention ensures the standardization of the data using the same measure.

Spectrophotometer

Method and system in optical microscopy based on the deflection of micro- and nanomechanical structures, upon impact of a laser beam thereon, which simultaneously and automatically provides a spatial map of the static deflection and of the form of various vibration modes, with vertical resolution in the subangstrom range. The invention comprises at least one mechanical structure, an incident laser beam sweeping the surface of the structure, an optometric detector for capturing the laser beam, and frequency excitation means that generate at least two sinusoidal signals at different frequencies in the mechanical structure.

Method and system for characterization of nano- and micromechanical structures

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Valerio Pini