Dow Agrosciences LLC

Agrigenetics, Inc.

Dianaplantsciences, Inc.

Vaka S Reddy

John P Davies

William M Ainley

Xing Liang Liu

Mark Allen Thompson

D Ry Wagner

Scott Bevan

Cory M Larsen

Helena V Mathews

Allan H Lammers

Patricia Ann Owens Merlo

Satyalinga S Gampala

Warren S Lee

Scott A Bevan

Xing L Liu

William Michael Ainley

Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4&#xd7; array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

Sugarcane bacilliform viral (SCBV) enhancer and its use in plant functional genomics

This disclosure concerns a system and method for detecting heterologous DNA in plant materials.

System and method for analysis of plant material for a set of unique exogenous genetic elements

An activation tagging construct for maize that includes one or more sugarcane bacilliform viral (SCBV) enhancer elements, and resulting tagged populations and plants, are described. In one example, an activation tagging DNA construct includes a coding sequence for a transposase, a detectable reporter (such as anthocyanin regulatory genes B-Peru and C1) and a non-autonomous transposable T-DNA cassette. For example, the transposable T-DNA cassette is inserted into the detectable reporter encoding region such that the B-Peru and C1 genes express anthocyanins in a cell containing the maize activation tagging DNA construct only upon excision of the transposable cassette. Methods of generating a tagged population of maize plants include transforming a maize plant cell or tissue with the disclosed constructs.

Sugarcane bacilliform viral enhancer-based activation tagging platform for maize, and resultant tagged populations and plants

Disclosed herein is an activation tagging construct for maize, resulting tagged populations and plants. In one example, an activation tagging DNA construct includes a coding sequence for a transposase, a detectable reporter (such as anthocyanin regulatory genes B-Peru and C1) and a non-autonomous transposable T-DNA cassette. For example, the transposable T-DNA cassette is inserted into the detectable reporter encoding region such that the B-Peru and C1 genes express anthocyanins in a cell containing the maize activation tagging DNA construct only upon excision of the transposable cassette. Methods of generating a tagged population of maize plants include transforming a maize plant cell or tissue with the disclosed constructs.

Activation tagging platform for maize, and resultant tagged populations and plants

Reported herein are methods for obtaining stably transformed callus in'Hicksii', including particularly methods that involve using needles, stem, or bark peel as explant material for transformation. Also provided are descriptions of several promoter activities in directing reporter gene expression incells, in particular cells in suspension cultures, callus and needles. Transgenic plants (e.g.,plants), plant cells, cell lines, and tissues (including seeds) are also provided, in particular those that express one or more enzymes in a paclitaxel biosynthesis pathway.

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Growing community of inventors

Aurora, CO, United States of America

Vaka S Reddy