Basf Se Corporation

Vaidotas Navickas

Alois Kindler

Michael Koch

Frank Rittig

Stefan Koch

Michael Breuer

Kai-Uwe Baldenius

Ferdinand Leifeld

Markus Gruen

Wolfgang Siegel

Michael Puhl

Melanie Weingarten

Petra Deckert

Bernd Nidetzky

Thomas Kaeding

Katrin Treier-Marxen

Andreas Schaedler

Martin Pfeiffer

Volker Wengert

Patricia Wildberger

The present invention is directed to a method of purifying a protein of interest from the particulate matter of a fermentation broth comprising the step of purifying the protein of interest from the particulate matter of the fermentation broth, wherein the step of purifying the protein of interest from the particulate matter of the fermentation broth comprises one or more washing steps, which comprises contacting the particulate matter with a washing solution comprising one or more conditions that favor the solubilization of the protein of interest and/or the desorption of the protein of interest from the particulate matter.

Method of purifying a protein from fermentation solids under desorbing conditions

The present invention relates to methods of producing sugar phosphates by enzymatic transphosphorylation, wherein the phosphoryl transfer from phosphate donor substrates to sugar substrates is catalyzed by an enzyme having alpha-glucose-1-phosphatase activity. Furthermore, the present invention relates to a biocatalyst having sucrose phosphorylase as well as alpha-glucose-1-phosphatase activity and its use. The production of an enzyme having alpha-glucose-1-phosphatase activity and its use in the transphosphorylation of sugar substrates is also presented herein.

Enzymatic transphosphorylation of sugar substrates

The present invention relates to biocatalytic methods, comprising purely enzymatic, mixed enzymatic-fermentative and purely fermentative methods, for the direct single-step conversion of L-fucitol to L-fucose, in order to easily obtain L-fucose at high amounts and levels of purity. Suitable recombinant microorganisms and fungi are further disclosed and also the use thereof in said method for the single-step conversion to L-fucose.

Biocatalytic production of l-fucose

Described is a method for processing cellulose-containing biomass with sulfuric acid and certain additives, especially for the pretreatment of cellulose-containing biomass prior to saccharification.

Method for processing cellulose-containing biomass

Described are a method for processing cellulose-containing biomass and the use of methanesulfonic acid for processing cellulose-containing biomass, especially for the pretreatment of cellulose-containing biomass prior to saccharification.

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Mannheim, Germany

Vaidotas Navickas