Massachusetts Institute of Technology

Uyanga Tsedev

Angela M Belcher

Fred Lam

Christopher A Voigt

Tae-Gon Cha

An engineered phage-derived particle (PDP) for expressing a transgene in a target cell transduced with a bacteriophage, the PDP includes (i) less than about 500 bp of DNA from the bacteriophage genome, (ii) an ITR-flanked therapeutic gene up to 20 kb, (iii) an endosomal escape sequence, (iv) a nuclear localization sequence, and (v) a cell-specific targeting moiety. The PDP may escape lysosomal degradation, traffic across the nuclear envelope and expressed a therapeutic gene in a mammalian cell.

M13 phage based gene therapy platform

Provided herein, in some aspects, are methods and compositions for producing single-stranded DNA (ssDNA) having uniform length.

Optimization of circular single stranded DNA using M13 phage

Provided are engineered phages populations, which are homogeneous in length, as well as methods of making and methods of using such phages. Also provided are engineered chlorotoxin-phages as well as their methods of making and using. The disclosed homogeneous phage populations and chlorotoxin-phages may be used, for example, for treating and/or imaging tumors, such as central nervous system tumors.

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Somerville, MA, United States of America

Uyanga Tsedev