Cranfield University

Poly-An Gesellschaft Zur Herstellung Von Polymeren Fuer Spezielle Anwendungen Und Analytik Mbh

Ploy-An Gesellschaft Zur Herstellung Von Polymeren Fuer Spezielle Anwendungen Und Analytik Mbh

Elipsa Gmbh

Poly-Am Gmbh

Uwe Schedler

Mathias Ulbricht

Jürgen Bendig

Pablo Wessig

Heike Matuschewski

Olena Volodimirivna Piletska

Sergiy Anatoliyovich Piletsky

Sergey A Piletsky

Anthony P F Turner

David C Cullen

David Weston

Tatinna Sergeyeva

Sergiy Piletski

The invention concerns a method for immobilizing biomolecules on a polymeric surface of a carrier material and a carrier material functionalized with a biomolecule according to the general formulas (IV) and (V), which can be produced according to the method, wherein P indicates the polymeric surface of the carrier material and M indicates the biomolecule bound to the linker compound via an amino group (formula IV) or a carbonyl group (formula V) and Rhas the meaning ORor NRR; R, Rand R, independently of one another, indicate H, and alkyl group or an aryl group; Rindicates H, an alkyl, an aryl, an acyl, an alkoxycarbonyl or an aryloxycarbonyl group; and the alkyl, aryl, acyl, alkoxycarbonyl and/or aryloxycarbonyl group of the radicals R, R, Rand R, independently of one another, are substituted or unsubstituted.

Functionalized carrier material containing covalently bound biomolecule via a linker

The invention concerns a novel surface-functionalized carrier material with a polymeric surface and at least one linker compound according to the general formula (I), which is covalently bound to the surface. In the formula, P indicates the polymeric surface; Rhas the meaning ORor NRRand R, Rand R, independently of one another, indicate H, an alkyl group or an aryl group; Rindicates H, an alkyl, an aryl, an acyl, an alkoxycarbonyl or an aryloxycarbonyl group; and the alkyl, aryl, acyl, alkoxycarbonyl and/or aryloxycarbonyl group of the radicals R, R, Rand R, independently of one another, are substituted or unsubstituted. The material according to the invention can be very easily produced by photochemical coupling and serves for the solid-phase synthesis of amino acids, peptides, proteins or molecules with at least one peptidic structural unit.

Surface-functionalized carrier material and solid phase synthesis method

The invention relates to a method for the production of a template-textured material by synthesis of a template-textured polymer (TTP) by performing crosslinking polymerization of functional monomers on a support in the presence of a template, which method is characterized in that a support having a thin polymer layer on the surface thereof is added with a reaction mixture consisting of polymerization initiator, template, functional monomer, crosslinker, solvent and/or buffer and, following sorption of the reaction mixture in the thin polymer layer, the polymerization is initiated and continued until the absorption capacity of the thin polymer layer for the template-textured polymer (TTP) is reached, and the template is optionally removed in a final step, the support used being selected in such a way that it cannot absorb the reaction solution. The materials of the invention are remarkable for their high binding specificity and selectivity for the template.

Method for producing template-textured materials with high binding specificity and selectivity and utilization of said materials

Reaction of a dialdehyde, particularly phthaldialdehyde (I), with R&mdash;Z where Z is a nucleophilic group (preferably SH) and R is polymerisable (e.g. allyl) gives a reactive thioacetal (V) which can react with an amine ligand L&mdash;NH to produce an isoindole (IV) which may be fluorescent. At some stage, generally before interaction with L&mdash;NH , the R groups are polymerised, possibly leading to self-assembly of the polymer on a metal or SH-bearing surface. Such a coated surface is useful as a transducer in assays or as a binding medium e.g. for chromatography.

Polymer for binding amine containing ligands and uses thereof

Template-textured materials in the form of template-textured polymers (TGP) on various surfaces including membranes (i.e, template-textured membranes). Such materials are created by modifying the surface of solid carriers, which, by cross-linking polymerization of functional monomers initiated on the surface of said solid carriers in the presence of a template, leads to stable template prints that subsequently bind template molecules or template derivatives in a specific manner. The invention also relates to methods for the production of TGPs and to the use thereof for substance-specific separation of materials.

Template-textured materials, methods for the production and use thereof

Growing community of inventors

Berlin, Germany

Uwe Schedler