Dsm IP Assets B.v.

Jiangnan University

Institut National De La Recherche Agronomique

Forskarpatent I Syd Ab

Evonik Degussa Gmbh

Uwe Sauer

Jian Chen

Oskar Zelder

Guocheng Du

Stefan Haefner

Jianghua Li

Corinna Klopprogge

Hans-Peter Hohmann

Long Liu

Andrea Herold

Nigel John Mouncey

Yanfeng Liu

Fredrik Wahlbom

Marco Sonderegger

Hannes Link

Stephane Aymerich

Jörg Mampel

Nicola Zamboni

Stéphane Aymerich

Hartwig SCHRÖDER

The present invention provides a method for enhancing N-acetylglucosamine production by usage of a recombinantwith a glcK knockout. This invention enhanced the production of GlcNAc by knocking out the glcK gene which encodes a glucokinase, thus eliminating the GlcNAc phosphorylation to GlcNAc-6-P. The specific growth rate and content of GlcNAc in the supernatant of the recombinantwith the glcK knockout were 0.15 hand 3.0 g/L, respectively, which were 2.32 times and 2.14 times of those of the control strain without glcK knockout. The recombinantof the present invention would be potentially useful for industrial production of GlcNAc.

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The present invention is directed to novel genes mediating the carbon catabolite repression (CCR) of gluconeogenic genes. Furthermore, the polypeptides encoded by said genes as well as the use of said genes in a process for the production of a target fermentation product is provided. Processes for generating such microorganisms are also provided by the present invention. The invention is also related to a genetically engineered microorganism and its use for the production of a target fermentation product, wherein the gluconeogenic genes are relieved from CCR within said microorganism.

Method for producing a fermentation product using a genetically modified microorganism with a mutation to relieve carbon catabolite repression

A process for the preparation of a target fermentation product comprising cultivation of an aerobic microorganism to produce such product, wherein in the microorganism the efficiency of the respiratory chain is increased, transformed and novel nucleotide sequences.

Fermentation process

The present invention relates to a method for preparing an ethanol producing, xylose utilizing strain ofcomprising genes for overexpression of xylose reductase, xylitol dehydrogenase and xylulokinase, wherein in addition to said genes for production o phosphoacetyltransferase, and acetaldehyde dehydrogenase are introduced and optionally overexpressed.

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Zurich, Switzerland

Uwe Sauer