Merck Patent Gmbh

Merck Patent Gesellschaft

Uwe Michelsen

Karl Holschuh

Joerg Von Hagen

Robertus Hendriks

Maria Wehsling

Jeorg Von Hagen

The present invention relates to the use of ionic liquids or of mixtures comprising at least one ionic liquid and at least one further solvent for the extraction of membrane proteins from biological samples, to methods for the extraction of membrane proteins, to a kit for the extraction of these proteins and to the use thereof.

Use of ionic liquids for membrane protein extraction

The present invention relates to a method for the extraction of proteins, protein fragments and/or peptides from biological samples, where the extractants employed are ionic liquids of the general formula KA, to a kit for the extraction of proteins, protein fragments and/or peptides, and to the use thereof.

Use of ionic liquids for protein extraction

The invention relates to a method of isolating plasmid DNA from microorganism cultures with the aid of solid-phase bodies. The solid-phase bodies can be silica gels, silicates, or glass-like materials and the solid-phase bodies can have magnetic properties. The solid-phase bodies are used for isolating the microorganisms and for isolating the plasmid DNA.

Method of isolating plasmid DNA

The invention provides a method for isolating and purifying nucleic acids from liquid samples. The method comprises using a solid carrier which is essentially made of oxide inorganic materials containing hydroxyl groups (e.g., silica gel or hydroxylapatite). The nucleic acids are bound to the carrier material in the acidic pH range while they are eluted in the alkaline pH range.

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Weinheim, Germany

Uwe Michelsen