Roche Diagnostics Operations, Inc.

Boehringer Mannheim Gmbh

Roche Molecular Systems, Inc.

Dako A/s

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Owlstone Medical Limited

Uwe Kobold

Dieter Heindl

Andreas Leinenbach

Martin Rempt

Christoph Seidel

Rupert Herrmann

Herbert Von Der Eltz

Hans-Peter Josel

Giuseppe Prencipe

Simon Ferdinand Loibl

Thomas Duelffer

Silvia Baecher

Jelena Milic

Roland Thiele

Anna-Skrollan Geiermann

Nicole Pirkl

Leopold Von Proff

Noah Weiss

Robert Hahn

Christian Klein

David H Gelfand

Frank Bergmann

Johann Karl

Erasmus Huber

Peter Sluka

Hartmut Duefel

Michael Grol

Bernd Vogt

Thomas Carell

Hannes Kuchelmeister

Benjamin Tiemann

Albert Geiger

Robert Lang

Michael Vogeser

Andreas Finke

Peter Kupser

Ivo Glynne Gut

Tobias Franz

Werner Naser

Thomas Heinemeyer

Holger Busskamp

Hubert Paul

Thomas Koch

Roderic Fuerst

Christoph Steinbeck

Toni Pfaffeneder

Angie Kirchner

Lauren Brown

Olesea Kosmatchev

Rene Rahimoff

Sarah Schiffers

Markus Muller

Harmut Duefel

Apostolos Kyriatsoulis

Susanne Feldmann

Hans-Werner Griesser

Eramus Huber

Doris Renauer

Nicole Horn

Angela Puhlmann

Thomas Hoffmann

Daniela Mazzier

Indranil Mitra

Christian Huber

Nathanael Delmotte

The present invention relates to reagents which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said reagents.

Reagent for mass spectrometry

The present invention relates to compounds which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said compounds.

Benzylpyridinium reagent for mass spectrometry

The present invention refers to a method for determining intact glycated hemoglobin A (HbA1c) by mass spectrometry (MS), a kit and a diagnostic system adapted for performing the method.

Automatic sample workflow for LC-MS based HbA1c measurement on intact protein level

A method for identifying a reagent during a process in an analysis system is disclosed. The analysis system comprises a liquid chromatograph and a mass spectrometer. The method comprises providing a reagent, adding at least one chemical substance to the reagent with a concentration being below a detection level of the mass spectrometer, enriching the chemical substance within the liquid chromatograph to a concentration above the detection level of the mass spectrometer, processing the reagent together with the enriched chemical substance by means of the analysis system, and identifying the reagent based on a detection of a substance detection signal of the mass spectrometer representing the chemical substance.

Method for identifying a reagent during a process in an analysis system

A method for identifying a reagent during a process in an analysis system is disclosed. The analysis system comprises a liquid chromatograph and a mass spectrometer. The method comprises providing a reagent, adding at least one chemical substance to the reagent with a concentration being above a detection level of the mass spectrometer, processing the reagent together with the chemical substance with the analysis system, and identifying the reagent based on a detection of a substance detection signal of the mass spectrometer representing the chemical substance.

The present disclosure is directed at an antibody conjugate having an antibody and a tag, wherein one or more element(s) present in the antibody exhibit an isotope ratio which differs from the naturally occurring isotope ratio of the one or more element(s), wherein the amount of the isotope which is less-common in nature, is increased to at least 4% of the atoms of the respective element in the antibody, as well as uses thereof.

Methods of protecting the sequence of an antibody conjugate from being determined

A method for detecting an analyte involving a) contacting the analyte, a capture agent and a solid surface to form a capture complex attached to a solid surface, b) cleaving at least one covalent bond within the analyte and/or within said capture agent in the capture complex and, thereby, releasing the analyte or a fragment thereof from the capture complex, and c) detecting at least one fragment of the analyte and, thereby, detecting the analyte is disclosed herein. A kit having i) a capture agent binding to an analyte; and ii) an agent cleaving at least one covalent bond within the analyte and/or within the capture agent; and to the use of a protease for releasing fragments of an analyte for detecting the analyte, wherein the analyte is bound to a solid surface via a capture agent is also disclosed herein.

Immobilized analytes

Copy protection for antibodies

The present invention relates to reagents suitable in the mass spectrometric determination of analyte molecules such as carbohydrates as well as adducts of such reagents and analyte molecules and applications of said reagents and adducts. Further, the present invention relates to methods for the mass spectrometric determination of analyte molecules.

A diagnostic system and method and an interconnected laboratory system comprising clinical diagnostic systems are presented. The diagnostic system comprises a sample preparation module, a liquid chromatography (LC) separation module coupled to the sample preparation module via a sample preparation/LC interface, a mass spectrometer (MS) module coupled to the LC separation module via an LC/MS interface, and a result calculation module for identifying and/or quantifying analytes or substances of interest contained in the samples and passed through the LC separation and MS modules. The diagnostic system comprises a controller programmed to monitor operational parameters (-) indicative of a performance status of the diagnostic system, to trigger a quality control procedure and/or a maintenance procedure whenever one or more parameters (-) of the operational parameters (-) is out of specification, and to minimize the quality control and/or maintenance procedures as long as the operational parameters (-) remains within specification.

Automated clinical diagnostic system and method

A method of quantifying the amount of at least two analytes A1 and A2 involving (a) adding at least one salt (S) to at least a portion (P1) of the sample comprising the at least two analytes A1 and A2, (b) ionizing at least a portion of the sample according to (a) thereby forming an analyte flow comprising the analytes A1 and A2 in ionized form, (c) separating the ionized analytes A1 and A2 from each other by using at least one ion mobility separator (), wherein the analyte flow according to (b) at least partially passes through the ion mobility separator (), and (d) quantifying the amount of the separated ionized analytes obtained according to (c), wherein A1 is a pharmaceutically active compound C or derivative thereof and A2 is a metabolite or stereoisomer of C.

Method and device for separating metabolites or stereoisomers

A method and an apparatus for determining a concentration of a target analyte in a sample of bodily fluid are disclosed. The method involves providing a sample of bodily fluid including the target analyte, providing an internal standard solution including a mixture of components having a plurality of isotopes of the target analyte, wherein a concentration of each isotope is unknown, adding the internal standard solution to the sample, analyzing the sample including the internal standard solution using a mass spectrometer, creating a sample function curve based on signal intensities, wherein the signal intensities define arbitrary units, transferring an analyte signal into a corresponding arbitrary analyte unit by means of the sample function curve, and transferring the arbitrary analyte unit into the concentration of a target analyte by means of a standardization function representing a curve of concentrations depending on the arbitrary units.

Method for determining a concentration of a target analyte in a sample of bodily fluid

A clinical diagnostic system is presented and comprises a sample preparation station for automatically preparing samples comprising analytes of interest, a liquid chromatography (LC) separation station comprising a plurality of LC channels and a sample preparation/LC interface for inputting prepared samples into the LC channels. The system further comprises a controller to assign samples to pre-defined sample preparation workflows each comprising a pre-defined sequence of sample preparation steps and requiring a pre-defined time for completion depending on the analytes. The controller further assigns an LC channel for each prepared sample depending on the analytes and plans an LC channel input sequence for inputting the prepared samples that allows analytes from different LC channels to elute in a non-overlapping LC eluate output sequence based on expected elution times. The controller further sets and initiates a start sequence that generates a prepared sample output sequence that matches the LC channel input sequence.

Automated clinical diagnostic system and method using parallel liquid chromatography channels and sample preparation workflows

Growing community of inventors

Weilheim, Germany

Uwe Kobold