Degussa Aktiengesellschaft

Uwe Eichhorn

Karlheinz Drauz

Andreas Bommarius

Hans-Dieter Jakubke

Matthias Kottenhahn

Peter Kuhl

The invention concerns a process for the enzymatic preparation of protected di- and oligopeptides and the separation of the protective groups used. The process according to the invention enables peptides to be synthesized simply and economically and the protective group to be separated carefully. The process comprises three reaction steps: 1. Preparation of N-carbamoyl amino acid or N-carbamoyl amino acid derivatives; 2. Formation of the peptide bond between the carbamoyl-protected electrophile and nucelophile; and 3. Separation of the carbamoyl-protective group.

Process for preparing peptides and N-carbamoyl-protected peptides

A water-supported enzymatic reaction is carried out with the substrate and the enzyme present in the solid phase. Either the water does not form a liquid continuum around substrate and enzyme, or the water acts at least partially out of the gas phase, or the water required for the reaction is added via a solid, or at least a part of the water required for the reaction is transferred by means of solid body contacts or by a combination of these means. The reaction mixture can be mixed by ultrasound or turbulence, e.g. in a fluidized bed, or both.

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Munich, Georgia

Uwe Eichhorn