Lonza Ag

Basf Aktiengesellschaft

Symrise Ag

Enzymicals Ag

Basf Se Corporation

Hoffmann-La Roche Inc.

Dsm IP Assets B.v.

Degussa Aktiengesellschaft

Symrise Gmbh & Co Kg

Frito-Lay Trading Compnay Gmbh

Brain Ag

Morphochem Ag

F. Hoffmann-La Roche Ag

B.r.a.i.n. Biotechnology Research and Information Network Ag

Micromun Institut Für Mikrobiologische Forschung Gmbh

Uwe Bornscheuer

Karen T Robins

Erik Henke

Jakob Peter Ley

Kai Doderer

Egon Gross

Matthias Höhne

Maren Thomsen

Dominique Böttcher

David Paul Weiner

Gerhard Krammer

Beat Wirz

Hans Iding

Tim S Hitchman

Jonathan D Lyon

Paul Spurr

Jürgen Eck

Thomas Scheper

Claudia Margarete Schmidt-Dannert

Klaus Liebeton

Ian-Lucas Gatfield

Harald Trauthwein

Steven Paul Hanlon

Sirirung Wongsakul

Jens-Michael Hilmer

Jens Michael Hilmer

Rolf Schmidt

Winfried Hinrichs

Ioannis Pavlidis

Martin Steffen Weiss

Mechthild Gall

Christin Peters

Patrick Jonczyk

Sascha Beutel

Peter Langer

Esen Bellur

Henrike Brundiek

Andrew Evitt

Stefan SaB

Friedericke Bonisch

Robert Kourist

Matthias Hoehne

Sebastian Schaetzle

Anke Hummel

Elke Brüsehaber

Dominique Boettcher

Elke Bruesehaber

Marlen Schmidt

Markus Kaehler

Andre Rieks

Anna Musidlowska

Stefan Lange

Ludger A Wessjohann

Gunther Scheid

Wouter Kuit

Romano Orru

Sandra Vorlov&aacute;

Hong Yang

Yang Hong

The invention relates to a method for producing flavone glycoside dihydrochalcones, having the following steps: (a) providing a transgenic microorganism containing (i) a first nucleic acid portion (A) containing a gene which codes for a bacterial chalcone isomerase and (ii) a second nucleic acid portion (B) containing a gene which codes for a bacterial enoate reductase, (b) adding one or more flavone glycosides to the transgenic microorganism under conditions which allow the simultaneous isomerization and reduction of the flavone glycoside into the flavone glycoside dihydrochalcone, and optionally (d) isolating and purifying the final product, wherein the nucleic acid portion (A) (1) is a nucleotide sequence according to SEQ ID NO:1, in which the nucleic acid portion (A') according to SEQ ID NO:3 has been cut out, or (2) is an amino acid sequence according to SEQ ID NO:2, in which the amino acid portion (A&#x2032;) according to SEQ ID NO:4 has been cut out.

Method for the biotechnological production of flavone glycoside dihydrochalcones

The present invention relates to a mutant transaminase with increased transaminase activity relative to the wild-type transaminase, a fusion protein comprising the transaminase, a polynucleotide coding for the transaminase, a host cell comprising the polynucleotide, mutant transaminase and/or fusion protein, a method of producing an amine with the mutant transaminase or fusion protein and the use of the mutant transaminase or fusion protein for the production of an amine.

Mutant transaminases as well as methods and uses relating thereto

A method for production of a dihydrochalcone, especially of phloretin, using a transgenic microorganism, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a'), comprising or consisting of a gene coding for a plant chalcone isomerase, and a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, corresponding transgenic microorganisms, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a&#x2032;), comprising or consisting of a gene coding for a plant chalcone isomerase, and/or a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, and host cells, containing one or more identical or different such vectors.

Method for biotechnological production of dihydrochalcones

The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phospholipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.

Hydrolases, nucleic acids encoding them and methods for making and using them

The present invention relates to a polynucleotide encoding an enzyme having carboxylesterase [E.C.3.1.1.1] activity.

Polynucleotides encoding enantioselective carboxylesterases and methods of making same

The present invention relates to the production of optically pure secondary amines, which can be used as intermediate products in a synthesis of for instance pharmaceutical products.

Process for the preparation of optically active chiral amines

The present invention relates to a newly identified lipase belonging to the Ustilaginaceae family of Basidomycetes and variants thereof. The invention also relates to polynucleotides encoding the lipase and the variants thereof. The invention further relates to procedures for producing the lipase, and variants thereof, polypeptides and polynucleotides. The invention further relates to lipase variants with an increased trans-selectivity. The invention further relates to lipase variants with a preference for long chain fatty acid esters or carboxylic acid moieties with a chain length greater than C. The invention further relates to a method of reducing, or eliminating, trans-fatty acids from liquid oil stable enough for frying systems.

Reduced-fat foodstuffs and cooking oils, and methods for making same

The present invention relates to the production of optically active amines, which can be used as intermediate products in a synthesis of for instance pharmaceutical products.

Process for preparation of optically active N-protected 3-aminopyrrolidine or optically active N-protected 3-aminopiperidine and the corresponding ketones by optical resolution of the racemic amine mixtures employing a bacterial omega-transaminase

The present invention relates to processes for the screening, preparation and characterization of (R)-selective &#x3c9;-transaminases, to transaminases obtained thereby and their uses in various transamination processes.

Process for the identification and preparation of a (R)-specific omega-transaminase

The invention relates to novel mutants of &#x3b3;PLE, to vehicles containing the same and to their use in the production of enantiomer-enriched alcohols, carboxylic acids and esters.

Isoforms of pig liver esterase

The present invention relates to a microorganism which comprises at least one copy of a polynucleic acid sequence which is foreign to the host and which encodes a protein having an enzymic activity, and comprises a chaperone system which assists the expression of the protein in the form of an active enzyme, and to a method for producing a protein having esterase activity using such a microorganism.

Microorganism for producing recombinant pig liver esterase

New enzymes having esterase activity and their use for processes for kinetic resolution of butinolesters.

Esterases and their use for processes for kinetic resolution of butinolesters

The invention relates to biotechnologically expressible, enzymically active recombinant porcine liver esterases, to a biotechnological method for the preparation thereof and to the use thereof in organic synthesis. The monomeric subunits of recombinant porcine liver esterase are truncated at their C-terminal end, compared with naturally occurring porcine liver esterase subunits. Moreover, it has proved to be an additional advantage to truncate the N-terminal end as well.

Recombinant porcine liver esterases, their use and a method for the production thereof

The present invention is directed to novel acyloins, their derivatives, methods for their production and their use for the production of novel epothilones and their derivatives. In addition, the invention is directed to the building blocks for epothilone synthesis, methods for their production and the use of synthetic building blocks for the production of epothilones and their derivatives.

EPOTHILONE SYNTHESIS BUILDING BLOCKS III AND IV: ASYMMETRICALLY SUBSTITUTED ACYLOINS AND ACYLOIN DERIVATIVES, METHODS FOR THEIR PRODUCTION AND METHODS FOR THE PRODUCTION OF EPOTHILONES B, D AND EPOTHILONE DERIVATIVES

L-Menthol is prepared from D,L-menthyl derivatives by enantioselective enzymatic cleavage.

Process for the preparation of L-menthol

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Greifswald, Germany

Uwe Bornscheuer