The Brigham and Women's Hospital, Inc.

Leland Stanford Junior University

The General Hospital Corporation

Beth Israel Deaconess Medical Center, Inc.

Worcester Polytechnic Institute

Purdue Research Foundation

Hillel LLC

Utkan Demirci

Waseem Asghar

Ronald W Davis

Lars Steinmetz

Naside Gozde Durmus

Huseyin Cumhur Tekin

Savas Tasoglu

Fatih Inci

Thiruppathiraja Chinnasamy

Mehmet Toner

Sanjiv Sam Gambhir

Daniel Irimia

Murat Baday

Xuanhong Cheng

Viswam Siva Nair

William Rodriguez

Ionita C Ghiran

Jaeyoung Yang

Alessandro Tocchio

Semih Calamak

Fei Liu

Erkan Tüzel

Antonio Carlos Sobieranski

Yi-Shao Liu

Rashid Bashir

Lee Zamir

Umut Atakan Gurkan

Hadi Shafiee

James Kingsley Leonard

Pu Chen

Sinan Güven

Selcuk Kilinc

Hooman Safaee

Derya A Yellin

Liju Yang

James Leonard Kingsley

Ionita Ghiran

Emre Kayaalp

Xiaohui Zhang

A heterogeneous population of cells are separated and collected according to a method. The heterogeneous population of cells in a paramagnetic medium are placed in a fluidic channel in which the fluidic channel comprises two or more outlets. The heterogeneous population of cells in the fluidic channel are separated based on differences in magnetic susceptibility and density of the heterogeneous population of cells. Fluid comprising the separated cells is withdrawn from the two or more outlets using variable flow rates by fluidic pumps at respective ones of the two or more outlets simultaneously to fractionalize the fluid comprising the separated cells across the two or more outlets by manipulation of the variable flow rates relative to one another.

Sorting biological and non-biological moieties using magnetic levitation

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

System and method for sperm sorting

A microfluidic chip is provided for self-sorting highly motile, morphologically normal sperm cell with high DNA integrity from a fresh semen sample. The sperm self-sorting microfluidic chip has one or more inlet chambers, and sperm collection outlet chamber(s), and the middle of the channel features various micro-fabricated structures in different geometrical shapes and orientations, with varying periodicities and patterns, such as an array of micro-fabricated pillars that facilitate the transport of the active and healthy sperm into the outlet chamber.

Micro-fluidic device for selective sorting of highly motile and morphologically normal sperm from unprocessed semen

Magnetic cell levitation and cell monitoring systems and methods are disclosed. A method for separating a heterogeneous population of cells is performed by placing a microcapillary channel containing the heterogeneous population of cells in a magnetically-responsive medium in the disclosed levitation system and separating the cells by balancing magnetic and corrected gravitational forces on the individual cells. A levitation system is also disclosed, having a microscope on which the microcapillary channel is placed and a set of two magnets between which the microcapillary channel is placed. Additionally, a method for monitoring cellular processes in real-time using the levitation system is disclosed.

System and method for cell levitation and monitoring

A device ('the ExoTIC device') for the isolation of extracellular vesicles from an extracellular vesicle-containing sample in which the sample is flowed through a membrane in a flow chamber to capture and purify the extracellular vesicles on the membrane. The extracellular vesicles may be washed and collected and utilized in any one of a number of ways including, but not limited to, identifying biomarkers of a disease, identifying the presence of a biomarker in a patient to determine whether a patient has a disease, and therapeutically treating existing diseases by re-introducing the extracellular vesicles, potentially modified, back into a body.

Exosome-total-isolation-chip (ExoTIC) device for isolation of exosome-based biomarkers

Systems and methods for levitating populations of moieties, cells, or other such units using one or more magnets in a microfluidic environment are provided. These systems and methods may be used to, for example, separate or sort heterogeneous populations of the units from one another, to assembly a multi-unit assembly during the levitating of the units, and to evaluate samples at the point of care in real-time. These systems and methods may also utilize a frame that enables an imaging device, such as a smartphone, to capture the units in real time as they are manipulated in the system.

Exosome-Total-Isolation-Chip (ExoTIC) device for isolation of exosome-based biomarkers

Growing community of inventors

Stanford, CA, United States of America

Utkan Demirci