Sungene Gmbh

Empresa Brasileira De Pesquisa Agropecuaria-embrapa

Basf Agrochemical Products B.v.

Sungene Gmbh & Co. Kgaa

Ute Linemann

Karin Herbers

Helke Hillebrand

Ulrich Keetman

Dale R Carlson

Tim Malefyt

Elibio Leopoldo Rech Filho

Carlos Alberto Arrabal Arias

Luiz Louzano

Bruce M Luzzi

Siyuan Tan

Adolfo Ulbrich

Tadashi Yotsumoto

Irene Kunze

Ulrich Keetmann

Compositions and methods related to transgenic AHAS-inhibiting herbicide resistant soybean plants are provided. Event 127 soybean plants having a mutated AHAS coding sequence which imparts tolerance to an AHAS-inhibiting herbicide are provided. The event 127 soybean plants having the event 127 nucleic acid molecule at the identified chromosomal location comprises genomic/transgene junctions having at least the nucleic acid sequence of SEQ ID NO:5 and/or 6. The characterization of the genomic insertion site of the event 127 provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event 127 soybean plants are provided.

Soybean event 127 and methods related thereto

Compositions and methods related to transgenic AHAS-inhibiting herbicide resistant soybean plants are provided. Event 127 soybean plants having a mutated AHAS coding sequence which imparts tolerance to an AHAS-inhibiting herbicide are provided. The event 127 soybean plants having the event 127 nucleic acid molecule at the identified chromosomal location may comprise genomic/transgene junctions having at least the nucleic acid sequence of SEQ ID NO: 5 and/or 6. The characterization of the genomic insertion site of the event 127 provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event 127 soybean plants are provided.

The present invention relates to expression cassettes comprising transcription regulating sequences with constitutive expression profiles in plants obtainable fromgenes At5g17920, At3g03780, At2g01100, At2g34770, At5g61560, At4g00830, At3g10220, At4g38520, At3g11110, At2g47170, Atlg64090, At5g60690, Atlg76350, At1g76580, Atlg31930, At5g18230, Atlg20970, or At4g35620. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such an expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector are also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with constitutive transcription regulating activity.

Constitutive expression cassettes for regulation of plant expression

The present invention relates to expression cassettes comprising transcription regulating sequences with constitutive expression profiles in plants obtainable fromgenes At5g17920, At3g03780, At2g01100, At2g34770, At5g61560, At4g00830, At3g10220, At4g38520, At3g11110, At2g47170, At1g64090, At5g60690, At1g76350, At1g76580, At1g31930, At5g18230, At1g20970, or At4g35620. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such an expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector are also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with constitutive transcription regulating activity.

The present invention relates to expression cassettes comprising transcription regulating sequences with root-preferential or root-specific expression profiles in plants obtainable fromgenes At1g66280, At1g74500, At1g49570, At1g70710, At5g66690, At3g29630, At5g48070, or At4g17800. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector is also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with root-specific or root-preferential transcription regulating activity.

Expression cassettes for root-preferential expression in plants

The invention relates to transgenic expression cassettes for expressing two nucleic acid sequences in a plant cell comprising at least one regulatory sequence selected from the group consisting of

Expression cassettes for the bi-directional transgenic expression of nucleic acids in plants

The present invention relates to expression cassettes comprising transcription regulating sequences with constitutive expression profiles in plants obtainable fromgenes At5g17920, At3g03780, At2g01100, At2g34770, At5g61560, At4g00830, At3g10220, At4g38520, At3g11110, At2g47170, At1g64090, At5g60690, At1g76350, At1g76580, At1g31930, At5g18230, At1g20970, or At4g35620.

The present invention relates to expression cassettes comprising transcription regulating sequences with meristem-preferential or meristem-specific expression profiles in plants obtainable fromgenes At2g02180, At5g54510, At2g26970, At2g01180, At3g45560, At4g00580, At1g54480, or At4g11490, or thegenomic sequences as described by SEQ ID NO: 35 or 36.

Expression cassettes for meristem-preferential expression in plants

The present invention relates to expression cassettes comprising transcription regulating sequences with root-preferential or root-specific expression profiles in plants obtainable fromgenes At1g66280, At1g74500, At1g49570, At1g70710, At5g66690, At3g29630, At5g48070, or At4g17800.

The present invention relates to expression cassettes comprising transcription regulating sequences with guard cell-preferential or guard cell-specific expression profiles in plants obtainable fromgene At5g58580, or fromgenomic DNA sequences as described by SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 12, 13, 14, or 15.

Growing community of inventors

Gatersleben, Germany

Ute Linemann