Council of Scientific and Industrial Research

Usha Yadav

Anil Kumar Singh

Suman Preet Singh Khanuja

Ajit Kumar Shasany

Mahendra Pandurang Darokar

Janak Raj Bahl

Sunita Dhawan

Raj Kishori Lal

Ravi Prakash Bansal

Om Parkash Dhawan

Ali Arif Naqvi

Rajender Singh Sangwan

Neelam Singh Sangwan

Alok Krishna

Virendra Kumar Singh Tomar

Bali Ram Tyagi

Soni Gupta

Avdhesh Kumar Srivastava

Sweta Pandey

Alok Kaira

The present invention was related to the development of a novel, distinct high yielding plant with rapid regeneration ability obtained through screening of the somaclones in a methodical way for fast regeneration in the tissue culture stage itself which was achieved by inventing the plant 'Kushal'. The plant yield higher herbage with corresponding high essential oil when evaluated with available superior varieties of mint in late planting condition during April when the fields are vacated after the harvest of Rabi crop like wheat, chickpea, coriander etc. Further the suckers required for commercial vegetative planting can be produced even in low land condition as the plant is reasonably tolerant to water logging compared to the best check 'Kosi'.

Mint plant 'Kushal' for late transplanting

The present invention relates to a method for profiling of protein extract from hyper-acidic aerial parts of plant Scented Geranium (sp.), said method comprising steps of selecting effective extraction medium, with extraction medium consisting of 0.2 M sodium carbonate increasing percentage extract by about 600%, selecting effective medium to determine polypeptide pattern in said extract, with polypeptide pattern medium consisting of Sodium Carbonate ranging between 0.2 to 0.4 M, and 0.2 M Sodium Carbonate along with 50% dimethylsulfoxide, selecting effective media to determine enzyme multimolecular forms in said extract, with enzyme multimolecular forms media consisting of 0.2 M Tris-HCl buffer at pH 7.5, and optionally 50% dimethylsulfoxide; Sodium Carbonate at concentration ranging between 0.2 to 0.4 M and optionally 50% dimethylsulfoxide, and using biochemical and biophysical techniques comprising gel electrophoresis, spectrophotometry, centrifugation, protein estimation in said method to determine quantitative, qualitative, structural and functional facets of the proteins from said extract, and extraction compositions thereof.

Protein profiling of hyper acidic plants and high protein extraction compositions thereof

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Lucknow, India

Usha Yadav