Harvard College

Molecular Loop Biosciences, Inc.

Uri Laserson

George M Church

Francois Vigneault

Gregory J Porreca

Ido Bachelet

This disclosure provides methods for monitoring an immune response. Methods comprise linking a polynucleotide sequence encoding a heavy chain variable region and a polynucleotide sequence encoding a light chain variable region from a single lymphocyte from a biological sample obtained before an immune response and linking a polynucleotide sequence encoding a heavy chain variable region and a polynucleotide sequence encoding a light chain variable region from a single lymphocyte from a biological sample obtained during or after an immune response. Methods further comprise performing high-throughput sequencing of the linked (paired) sequences from before the immune response and from during or after the immune response, and comparing the resulting sequence reads.

High-throughput immune sequencing

Aspects of the invention relates to methods and compositions that are useful to reduce bias and increase the reproducibility of multiplex analysis of genetic loci. In some configurations, predetermined preparative steps and/or nucleic acid sequence analysis techniques are used in multiplex analyses for a plurality of genetic loci in a plurality of samples.

Methods and compositions for evaluating genetic markers

Methods and compositions for determining and/or monitoring the immune state of an individual are provided.

Growing community of inventors

Boston, MA, United States of America

Uri Laserson