Life Technologies Corporation

Abt Holding Company

University of Minnesota

Uma Lakshmipathy

Rene Quintanilla

Catherine M Verfaillie

Joanna Asprer

Kyle R Gee

Mahendra Rao

Upinder Singh

Scott Grecian

Shannon Buckley

Marta Serafini

Provided herein are compositions, methods and uses that relate to or result from the identification of markers that can distinguish between cells at different stages of pluripotency. Certain embodiments provide markers that can distinguish between parental cells (i.e. differentiated cells), partially pluripotent (i.e. partially reprogrammed) and pluripotent (i.e. fully reprogrammed cells). Also provided here are uses of such differential markers, for example, in identification of cell potential, in diagnostics, in differential separation, and in creating efficient workflows that involve fewer steps and lesser time in identifying or separating a desired reprogrammed clone or cell line from a mixture of cells at various stages of pluripotency. In certain embodiments, the activity of these markers can be manipulated to influence cell potential for research or medical purposes.

Markers capable of distinguishing cell pluripotency and uses thereof

The invention relates to methods of altering gene expression by homologous recombination in a multipotent adult progenitor cell (MAPC). In particular, methods of producing a recombinant MAPC, of correcting a genetic defect in a mammal, of providing a functional and/or therapeutic protein to a mammal, and of transforming a MAPC are provided. MAPCs containing an erogenous DNA as well as recombinant MAPCs and their differentiated progeny are also provided.

Homologous recombination in multipotent adult progenitor cells

The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.

Substrates and methods for staining live stem cells

Growing community of inventors

Carlsbad, CA, United States of America

Uma Lakshmipathy