Roche Diagnostics Operations, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Ulrike Fischer

Horst Menzler

Hans Schels

Harald Sobek

Johann-Peter Thalhofer

Alfons Nichtl

Michael Greif

Manfred Watzele

Gerhard Hiller

Bernd Buchberger

Martina H&ouml;sch

J&uuml;rgen Klepp

The present invention relates to a formulation of a thermostable DNA polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (PCR). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step.

Detergent free polymerases

The invention is a multichamber microdialysis device with a multitude of sample chambers in close side by side arrangement (similar to a microtiter plate) for receiving liquid samples, and at least one dialysate chamber for receiving a dialysate liquid. The sample chambers are surrounded by circumferential sidewalls and are in liquid exchange contact to a neighboring dialysis chamber via an exchange opening covered by a semipermeable membrane. The semipermeable membrane is fixed liquid-tight to the walls of the sample chamber, so that a diffusion exchange between the sample chamber and the correspondingly neighboring dialysate chamber is only possible via the membrane. Consequently, only molecules with a molecular weight below the molecular cut-off of the semipermeable membrane can diffuse from the sample chamber to the dialysate chamber or from the dialysate chamber to the sample chamber.

Multichamber microdialysis device

General purpose structure of an analytical element and its application for analyte determination

Bioreaction module for biochemical reactions

Growing community of inventors

Penzberg, Germany

Ulrike Fischer